Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Basic Med Sci. 2014 Sep;17(9):716-21.
Asthma results from the interaction between genetic and environmental factors. ADAM33 gene on chromosome 20p13 is associated with asthma and airway hyperresponsiveness.
This is a case-control study, where four SNPs S1 (rs3918396), T1 (rs2280091), T2 (rs2280090), V4 (rs2787094) of ADAM33 gene have been assessed in patients with allergic asthma and normal controls (95 patients and 86 normal). Blood samples of these participants have been genotyped by PCR and the RFLP method.
There was no association between asthmatic patients and polymorphisms of alleles, genotypes and haplotypes of the ADAM33 gene. When categorizing the asthmatic patients in severe, moderate and mild groups, associations in the subcategories of asthmatic patients were found. There were associations between polymorphisms of C allele of T1 SNP with severe asthmatic patients and G allele of V4 SNP with moderate asthmatics respectively (P=0.006, P=0.01). There was a significant association between sensitivity to mite and polymorphism of C allele of T1 SNP (P=0.02). Besides, there was a significant association between sensitivity to weeds and genotype GG of V4 SNP (P=0.05).
Polymorphisms of ADAM33 gene might be associated with severe asthma and sensitivity to aeroallergens in northeast of Iran, but further studies are needed to determine the polymorphisms in this area and other regions of our country.
哮喘是遗传和环境因素相互作用的结果。20p13 染色体上的 ADAM33 基因与哮喘和气道高反应性有关。
这是一项病例对照研究，评估了 ADAM33 基因的四个 SNP（S1(rs3918396)、T1(rs2280091)、T2(rs2280090)、V4(rs2787094)）在过敏性哮喘患者和正常对照组（95 例患者和 86 例正常对照）中的作用。通过 PCR 和 RFLP 方法对这些参与者的血液样本进行基因分型。
哮喘患者与 ADAM33 基因等位基因、基因型和单倍型的多态性之间没有关联。当将哮喘患者分为严重、中度和轻度组时，发现哮喘患者亚组存在关联。T1SNP 的 C 等位基因多态性与严重哮喘患者，V4SNP 的 G 等位基因多态性与中度哮喘患者之间存在关联（P=0.006，P=0.01）。T1SNP 的 C 等位基因多态性与螨敏感性之间存在显著关联（P=0.02）。此外，V4SNP 的 GG 基因型与杂草敏感性之间存在显著关联（P=0.05）。
ADAM33 基因的多态性可能与伊朗东北部的严重哮喘和对气传变应原的敏感性有关，但需要进一步研究以确定该地区和我国其他地区的多态性。
