Human Genetics Laboratory, Department of Studies in Zoology, University of Mysore, Manasagangothri, Mysore 570 006, India.
Lung. 2010 Oct;188(5):415-22. doi: 10.1007/s00408-010-9247-2. Epub 2010 Jun 4.
There are more than 100 candidate genes of asthma located on 23 human chromosomes. Interleukin-4 (IL-4), located on chromosome 5q31, and ADAM33, located on chromosome 20p13, and some single nucleotide polymorphisms (SNPs) of these genes have been shown to be associated with asthma and its manifestations in different populations. The most prominent SNPs of IL-4 and ADAM33 are 589C>T and 400A>G, respectively. There are also controversial reports on the association of these SNPs with asthma. In the present study, we analyzed these two SNPs in 100 patients with asthma and 50 controls through PCR amplification and restriction digestion to evaluate association of these two SNPs with asthma. The nonsignificant differences were observed for the IL-4 promoter polymorphism C589T and the ADAM33 T1 polymorphism between asthmatic patients and controls (P = 0.638 and 0.943, respectively). Our data revealed that there is no association of these SNPs with asthma indicating that other SNPs of these genes or other genes might be involved in the manifestation of asthma.
哮喘的 100 多个候选基因位于 23 条人类染色体上。白细胞介素-4（IL-4）位于 5q31 染色体上，ADAM33 位于 20p13 染色体上，这些基因的一些单核苷酸多态性（SNP）已被证明与不同人群的哮喘及其表现有关。IL-4 和 ADAM33 最突出的 SNP 分别为 589C>T 和 400A>G。这些 SNP 与哮喘的相关性也存在争议报道。在本研究中，我们通过 PCR 扩增和限制性消化分析了 100 例哮喘患者和 50 例对照者中的这两个 SNP，以评估这两个 SNP 与哮喘的相关性。哮喘患者和对照组之间，IL-4 启动子多态性 C589T 和 ADAM33 T1 多态性无显著差异（P 值分别为 0.638 和 0.943）。我们的数据表明，这些 SNP 与哮喘无关，提示这些基因或其他基因的其他 SNP 可能参与了哮喘的表现。
