Niewiarowska K, Pryczynicz A, Dymicka-Piekarska V, Gryko M, Cepowicz D, Famulski W, Kemona A, Guzińska-Ustymowicz K
Katarzyna Niewiarowska, Department of General Pathomorphology, Medical University of Bialystok, Waszyngtona 13, 15-269 Bialystok, Poland, tel. +48 85 748 59 42, fax +48 85 748 59 96, e-mail:
Pol J Pathol. 2014 Dec;65(4):296-304. doi: 10.5114/pjp.2014.48191.
Tissue inhibitor of metalloproteinase-1 (TIMP-1) inhibits the ability of cancer cells to metastasize, but it can also stimulate cancer development. The aim of this study was to assess the level of TIMP-1 in serum and its expression in patients with colorectal cancer (CRC). The study group consisted of 43 patients diagnosed with colorectal cancer and 24 healthy volunteers. The level of TIMP-1 was assessed by the ELISA method while the expression of this protein was performed immunohistochemically. The concentration of TIMP-1 in the sera of colorectal cancer patients was significantly higher than in the healthy control group (p = 0.004). Higher level of TIMP-1 in the sera correlated with female gender (p = 0.045), tumor location in colon (p = 0.016), poorly differentiated tumor (p = 0.034) and higher platelet count in whole blood (p < 0.004). A positive reaction of the protein in cancer cells was observed in 31 cases and was found to correlate negatively with its reaction in peritumoral stroma (p < 0.001). According to this study, TIMP-1 protein may play an important role in cancer development. The assessment of this molecule in serum and tissue can be useful at the time of diagnosis and can help us to understand the nature of colorectal pathogenesis.
基质金属蛋白酶-1组织抑制剂（TIMP-1）可抑制癌细胞转移能力，但也能刺激癌症发展。本研究旨在评估血清中TIMP-1水平及其在结直肠癌（CRC）患者中的表达。研究组由43例诊断为结直肠癌的患者和24名健康志愿者组成。采用ELISA法评估TIMP-1水平，同时通过免疫组织化学法检测该蛋白的表达。结直肠癌患者血清中TIMP-1浓度显著高于健康对照组（p = 0.004）。血清中TIMP-1水平较高与女性性别（p = 0.045）、肿瘤位于结肠（p = 0.016）、肿瘤分化差（p = 0.034）以及全血中血小板计数较高（p < 0.004）相关。在31例病例中观察到癌细胞中该蛋白呈阳性反应，且发现其与肿瘤周围基质中的反应呈负相关（p < 0.001）。根据本研究，TIMP-1蛋白可能在癌症发展中起重要作用。在诊断时评估血清和组织中的该分子可能有用，并有助于我们了解结直肠癌发病机制的本质。
