D'Eustachio P, Fein B, Michaelson J, Taylor B A
J Exp Med. 1984 Mar 1;159(3):958-63. doi: 10.1084/jem.159.3.958.
DNA sequences homologous to adult alpha-globin genes are dispersed in the mouse. Two functional genes are tightly linked on chromosome 11. Pseudogenes have been assigned to chromosomes 15 and 17 by analysis of interspecies somatic cell hybrids. We have now further characterized the second of these pseudogenes, Hba-a4. The gene is highly polymorphic, with three forms occurring in a panel of 15 inbred strains and a fourth occurring in an inbred strain derived from M. m. molossinus. Analysis of Hba-a4 alleles in CXB, BXH, and AKXL recombinant inbred strains placed Hba-a4 6.60 +/- 3.14 cM centromeric to H-2. Analysis of congenic mouse strains confirmed the linkage and the gene order. Hba-a4 is the first mammalian dispersed pseudogene to be localized in a linkage map, and should provide a useful marker for the region of chromosome 17 proximal to H-2.
与成年α-珠蛋白基因同源的DNA序列分散在小鼠体内。两个功能基因紧密连锁在11号染色体上。通过种间体细胞杂种分析,假基因已被定位到15号和17号染色体上。我们现在进一步对这些假基因中的第二个Hba-a4进行了特征描述。该基因具有高度多态性,在一组15个近交系中出现了三种形式,在源自小家鼠的一个近交系中出现了第四种形式。对CXB、BXH和AKXL重组近交系中Hba-a4等位基因的分析将Hba-a4定位在H-2着丝粒方向6.60±3.14 cM处。对同源近交系小鼠的分析证实了这种连锁关系和基因顺序。Hba-a4是第一个被定位到连锁图谱中的哺乳动物分散假基因,应该为17号染色体上H-2近端区域提供一个有用的标记。