一种用于检测呼吸道样本中人类肠道病毒68型的新型实时逆转录聚合酶链反应检测方法。
A new real-time reverse transcription-PCR assay for detection of human enterovirus 68 in respiratory samples.
作者信息
Piralla Antonio, Girello Alessia, Premoli Marta, Baldanti Fausto
机构信息
Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy Section of Microbiology, Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy
出版信息
J Clin Microbiol. 2015 May;53(5):1725-6. doi: 10.1128/JCM.03691-14. Epub 2015 Feb 18.
Abstract
A global reemergence of human enterovirus 68 (EV-D68) associated with severe respiratory illness occurred in 2014. We developed and validated an EV-D68-specific real-time reverse transcription-PCR (RT-PCR) for the detection of EV-D68 in respiratory samples. The rapid diagnosis of EV-D68 may contribute to better management of EV-D68 infections.
摘要
2014年，与严重呼吸道疾病相关的人类肠道病毒68型（EV-D68）在全球再度出现。我们开发并验证了一种用于检测呼吸道样本中EV-D68的特异性实时逆转录聚合酶链反应（RT-PCR）。EV-D68的快速诊断可能有助于更好地管理EV-D68感染。
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