Department of Virology, Norwegian Institute of Public Health, Oslo, Norway.
Influenza Other Respir Viruses. 2015 Mar;9(2):59-63. doi: 10.1111/irv.12300. Epub 2014 Dec 23.
An unexpectedly high proportion of children were admitted for severe respiratory infections at the Oslo University Hospital, Ullevål, Norway, during September and October, 2014. In light of the ongoing outbreak of enterovirus-D68 (EV-D68) in North America a real-time RT-PCR for screening of enterovirus and enterovirus D68 was established.
We developed a duplex real-time RT-PCR for rapid screening of enterovirus D68. The method target the 5' non-translated region (NTR) of the HEV genome at a location generally used for enterovirus detection.
Nasopharyngeal samples (n = 354), from children <15 years of age, received for respiratory virus analysis in OUH during September 1st and October 31nd, 2014, were tested for enterovirus and screened for enterovirus D68.
The duplex real-time RT-PCR method was an efficient tool for rapid screening for EV-D68 in respiratory specimens. Enterovirus was detected in 66 (22%) of 303 pediatric nasopharyngeal samples collected from children hospitalised with acute respiratory infection within the two-month period. Out of these, 33 (50%) were EV-D68. EV-D68 was associated with acute flaccid paralysis in one child.
An unexpectedly high proportion of children admitted for severe respiratory infections at the Oslo University Hospital, Ullevål, Norway, were diagnosed with EV- D68 during September 1st and October 31nd, 2014. These results emphasise that greater vigilance is required throughout Europe as enteroviruses are cause of severe respiratory disease.
2014 年 9 月至 10 月，挪威奥斯陆大学医院收治了大量患有严重呼吸道感染的儿童，比例之高出人意料。鉴于北美正在爆发肠道病毒 D68（EV-D68），我们建立了一种实时 RT-PCR 方法，用于筛查肠道病毒和 EV-D68。
我们开发了一种用于快速筛查 EV-D68 的双重实时 RT-PCR 方法。该方法针对 HEV 基因组 5'非翻译区（NTR）的一个位置，该位置通常用于肠道病毒检测。
2014 年 9 月 1 日至 10 月 31 日，在奥斯陆大学医院接受呼吸道病毒分析的<15 岁儿童鼻咽样本（n = 354），用于检测肠道病毒，并筛查 EV-D68。
该双重实时 RT-PCR 方法是一种快速筛查呼吸道标本中 EV-D68 的有效工具。在 303 例因急性呼吸道感染住院的儿童鼻咽样本中，有 66 例（22%）检测到肠道病毒，其中 33 例（50%）为 EV-D68。EV-D68 与 1 例急性弛缓性麻痹有关。
2014 年 9 月 1 日至 10 月 31 日，在挪威奥斯陆大学医院因严重呼吸道感染住院的儿童中，有相当高比例的儿童被诊断为 EV-D68。这些结果强调，欧洲各地需要保持高度警惕，因为肠道病毒是严重呼吸道疾病的病因。
