Korbekandi Hassan, Chitsazi Mohammad Reza, Asghari Gholamreza, Bahri Najafi Rahim, Badii Akbar, Iravani Siavash
Department of Genetics & Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences , Isfahan , Iran .
Pharm Biol. 2015 Jun;53(6):807-12. doi: 10.3109/13880209.2014.942868. Epub 2015 Feb 20.
There is an ever-growing need to develop green, non-toxic, and eco-friendly procedures for synthesis and assembly of nanoparticles (NPs) with the desired morphologies and sizes. The hydroalcoholic extract of Persian oak leaves [Quercus brantii Lindl. (Fagaceae)] contains high content of phenolic and flavonoid compounds with strong antioxidant activities, and it seems that this plant can be considered a good candidate for metal nanoparticle synthesis.
The potential of Q. brantii leaves in the production of silver NPs and the effect of the extract ethanol concentration on the produced NPs were studied.
Quercus brantii leaves were freshly collected, air-dried at room temperature, powdered, and sieved. Hydroalcoholic extracts (70% and 96%) were prepared by percolation of the plant powder. The reaction mixtures contained (final concentrations): AgNO3 (1 mM) as the substrate, plant extract as the biocatalyst, and phosphate buffer (pH = 7, 100 mM) as the reaction medium. Silver ions were determined using atomic absorption analysis. Particle size distribution of NPs was analyzed using Nano-Zeta Sizer (Malvern Instruments Ltd, Malvern, UK). Samples for TEM were prepared by drop-coating the silver nanoparticle suspensions onto carbon-coated copper grids.
Hydroalcoholic extract (96%) of Q. brantii successfully produced quite small (as small as 0.83 nm and the mean size of 6 nm), spherical, and poly-dispersed NPs with low aggregates. The conversion was fast and completed in 5 h.
This plant and the extraction method seem to be quiet attractive for industrial scale production of NPs.
开发绿色、无毒且环保的方法来合成和组装具有所需形态和尺寸的纳米颗粒（NPs）的需求日益增长。波斯橡树树叶[Quercus brantii Lindl.（壳斗科）]的水醇提取物含有高含量的具有强抗氧化活性的酚类和黄酮类化合物，并且这种植物似乎可被视为金属纳米颗粒合成的良好候选者。
研究了波斯橡树树叶在生产银纳米颗粒方面的潜力以及提取物乙醇浓度对所产生的纳米颗粒的影响。
新鲜采集波斯橡树树叶，在室温下风干、研磨并过筛。通过对植物粉末进行渗滤制备水醇提取物（70%和96%）。反应混合物包含（终浓度）：作为底物的硝酸银（1 mM）、作为生物催化剂的植物提取物以及作为反应介质的磷酸盐缓冲液（pH = 7，100 mM）。使用原子吸收分析法测定银离子。使用纳米Zeta粒度分析仪（英国马尔文马尔文仪器有限公司）分析纳米颗粒的粒度分布。通过将银纳米颗粒悬浮液滴涂到碳涂覆的铜网上制备用于透射电子显微镜（TEM）的样品。
波斯橡树树叶的96%水醇提取物成功制备出相当小（小至0.83 nm且平均尺寸为6 nm）、球形且多分散的纳米颗粒，聚集体较少。转化迅速，在5小时内完成。
这种植物及其提取方法对于纳米颗粒的工业规模生产似乎颇具吸引力。
