Wen Lu, Zeng Pei, Zhang Liping, Huang Wenli, Wang Hui, Chen Gang
Department of Pharmaceutics, School of Pharmacy, Guangzhou, People's Republic of China.
School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.
Int J Nanomedicine. 2016 Jun 10;11:2757-67. doi: 10.2147/IJN.S106662. eCollection 2016.
In this study, silver nanoparticles (AgNPs) were synthesized for the first time using an antibacterial endophytic fungus of Chinese medicinal herb Orchidantha chinensis, which has anti-inflammatory and antimicrobial activities. The AgNPs were analyzed by various characterization techniques to reveal their morphology, chemical composition, and stability. Also, the relationship between Chinese medicinal herbs, endophytic fungi, and the property of AgNPs was investigated for the first time. Interestingly, an experiment performed in this study revealed the proteins produced by the endophytic fungus to be capped on the nanoparticles, which led to an increase in the stability of spherical and polydispersed AgNPs with low aggregation for over 6 months. More importantly, further study demonstrated that the AgNPs possessed superior antibacterial activity and effectively promoted wound healing. Altogether, the biosynthesis of active AgNPs using the endophytic fungus from Chinese medicinal herb based on the symbiosis theory is simple, eco-friendly, and promising.
在本研究中，首次利用具有抗炎和抗菌活性的中国药用植物中华虎耳草的一种抗菌内生真菌合成了银纳米颗粒（AgNPs）。通过各种表征技术对AgNPs进行分析，以揭示其形态、化学成分和稳定性。此外，还首次研究了中草药、内生真菌与AgNPs性质之间的关系。有趣的是，本研究中进行的一项实验表明，内生真菌产生的蛋白质包裹在纳米颗粒上，这导致球形且多分散的低聚集AgNPs的稳定性增加超过6个月。更重要的是，进一步研究表明，AgNPs具有优异的抗菌活性，并能有效促进伤口愈合。总之，基于共生理论利用中草药内生真菌生物合成活性AgNPs简单、环保且前景广阔。
