Salturk Ziya, Çakır Çağlar, Sünnetçi Gürcan, Atar Yavuz, Kumral Tolgar Lütfi, Yıldırım Güven, Berkiten Güler, Uyar Yavuz
Okmeydanı Training and Research Hospital, ENT Clinic, Istanbul, Turkey.
Okmeydanı Training and Research Hospital, Pathology Clinic, Istanbul, Turkey.
J Voice. 2015 Sep;29(5):560-3. doi: 10.1016/j.jvoice.2014.10.013. Epub 2015 Feb 19.
We aimed to assess the effects of electronic nicotine delivery system (ENDS) or also termed electronic cigarette vapor on the laryngeal mucosa of rats.
Sixteen female Wistar albino rats were divided into two groups. The study group was exposed to ENDS vapor for 1 hour/day for 4 weeks. The control group was not subjected to any chemical or physical stimulus. The vocal folds of the study and control group rats were evaluated histopathologically by hematoxylin and eosin staining and immunohistochemically by Ki67 staining. Epithelial distribution, inflammation, hyperplasia, and metaplasia were evaluated.
Epithelial distribution and inflammation did not differ between the two groups. Two cases of hyperplasia were detected in the study group but there was no hyperplasia in the control group. Four cases of metaplasia were detected in the study group and one case in the control group. Statistical analysis revealed no significant difference between the study and control groups (P = 0.131 and 0.106, respectively).
Exposure to ENDS for 4 weeks caused hyperplasia and metaplasia of the laryngeal mucosa of rats but this was not significant statistically. These results implemented that further studies with larger cohort and longer duration are required to evaluate long-term effects.
我们旨在评估电子尼古丁传送系统（ENDS）或也称为电子烟蒸汽对大鼠喉黏膜的影响。
16只雌性Wistar白化大鼠被分为两组。研究组每天暴露于ENDS蒸汽1小时，持续4周。对照组未受到任何化学或物理刺激。通过苏木精和伊红染色对研究组和对照组大鼠的声带进行组织病理学评估，并通过Ki67染色进行免疫组织化学评估。评估上皮分布、炎症、增生和化生情况。
两组之间上皮分布和炎症无差异。研究组检测到2例增生，但对照组未出现增生。研究组检测到4例化生，对照组检测到1例化生。统计分析显示研究组和对照组之间无显著差异（P值分别为0.131和0.106）。
暴露于ENDS 4周导致大鼠喉黏膜增生和化生，但在统计学上不显著。这些结果表明需要进行更大样本量和更长时间的进一步研究来评估长期影响。
