Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Bioresour Technol. 2015 Apr;182:289-295. doi: 10.1016/j.biortech.2015.02.009. Epub 2015 Feb 11.
The effect of γ-irradiation pretreatment was compared with other pretreatment methods including ionic liquids (ILs), 1% HCl, 1% H2SO4, acidic aqueous Ils (AA-ILs), on the bioconversion efficiency of microcrystalline cellulose (MCC) for bioethanol production. The efficiency of MCC pretreatment followed with simultaneous saccharification and fermentation (SSF) was firstly evaluated according to the variations of the irradiation-derived compounds and structure of MCC, as well as yeast growth curve and bioethanol yield. Results showed that the appropriate irradiation dose (891 kGy used in our work) could eliminate the negative effect of toxic irradiation-derived compounds on SSF for ethanol bioconversion with the yield value of 67%. Analyses of SEM, FT-IR, reducing sugar and bioethanol yield showed that the efficiency of pretreatment on MCC was ILs ≈ irradiation pretreatment > AA-ILs pretreatment > 1% HCl pretreatment > 1% H2SO4 pretreatment.
γ-辐照预处理的效果与其他预处理方法（包括离子液体（ILs）、1% HCl、1% H2SO4、酸性水相离子液体（AA-ILs））进行了比较，这些方法均应用于微晶纤维素（MCC）的生物乙醇生产中的生物转化效率。首先根据辐照衍生化合物和 MCC 结构的变化、酵母生长曲线和生物乙醇产率，评估了 MCC 预处理后同步糖化发酵（SSF）的效率。结果表明，适当的辐照剂量（本工作中使用的 891 kGy）可以消除有毒辐照衍生化合物对 SSF 乙醇生物转化的负面影响，产率值为 67%。SEM、FT-IR、还原糖和生物乙醇产率分析表明，MCC 预处理的效率为 ILs≈辐照预处理＞AA-ILs 预处理＞1% HCl 预处理＞1% H2SO4 预处理。
