• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于检测犊牛源大肠杆菌中K99菌毛抗原的甘露糖抗性血凝试验、酶联免疫吸附测定和免疫电子显微镜技术

Mannose-resistant hemagglutination, enzyme-linked immunosorbent assay, and immune electron microscopy for detection of K99 fimbrial antigen in Escherichia coli from calves.

作者信息

Hernández F, Caballero M, Rivera P, Hird D

机构信息

Centro de Investigación y Diagnostico en Parasitologia, Facultad de Microbiologia, Universidad de Costa Rica.

出版信息

J Clin Microbiol. 1989 Sep;27(9):2123-4. doi: 10.1128/jcm.27.9.2123-2124.1989.

DOI:10.1128/jcm.27.9.2123-2124.1989
PMID:2570792
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC267755/
Abstract

Mannose-resistant hemagglutination (MRHA) was evaluated for identification of Escherichia coli with K99 fimbriae. The sensitivity and specificity of MRHA, relative to the enzyme-linked immunosorbent assay, were 21 and 79%, respectively. Disagreement between the tests may have been due in part to separation of pili from the cells, with resultant enzyme-linked immunosorbent assay-positive, MRHA-negative tests. MRHA was not useful as the sole means for the identification of E. coli with K99 fimbriae.

摘要

评估甘露糖抗性血凝反应（MRHA）用于鉴定具有K99菌毛的大肠杆菌。相对于酶联免疫吸附测定，MRHA的敏感性和特异性分别为21%和79%。两种检测方法之间的不一致可能部分归因于菌毛与细胞分离，导致酶联免疫吸附测定呈阳性而MRHA呈阴性。MRHA作为鉴定具有K99菌毛大肠杆菌的唯一方法并不实用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd9b/267755/98e2718cdaaf/jcm00069-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd9b/267755/98e2718cdaaf/jcm00069-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd9b/267755/98e2718cdaaf/jcm00069-0228-a.jpg

相似文献

1
Mannose-resistant hemagglutination, enzyme-linked immunosorbent assay, and immune electron microscopy for detection of K99 fimbrial antigen in Escherichia coli from calves.用于检测犊牛源大肠杆菌中K99菌毛抗原的甘露糖抗性血凝试验、酶联免疫吸附测定和免疫电子显微镜技术
J Clin Microbiol. 1989 Sep;27(9):2123-4. doi: 10.1128/jcm.27.9.2123-2124.1989.
2
Monoclonal antibody passive hemagglutination and capture enzyme-linked immunosorbent assays for direct detection and quantitation of F41 and K99 fimbrial antigens in enterotoxigenic Escherichia coli.用于直接检测和定量产肠毒素大肠杆菌中F41和K99菌毛抗原的单克隆抗体被动血凝试验和捕获酶联免疫吸附测定
J Clin Microbiol. 1987 Feb;25(2):278-84. doi: 10.1128/jcm.25.2.278-284.1987.
3
[ELISA for the detection of K99 fimbrial antigen of enterotoxic E. coli in feces].[酶联免疫吸附测定法检测粪便中肠毒素性大肠杆菌的K99菌毛抗原]
Schweiz Arch Tierheilkd. 1984 Dec;126(12):607-20.
4
Characterization of a new fimbrial antigen present in Escherichia coli strains isolated from calves.对从犊牛分离出的大肠杆菌菌株中存在的一种新型菌毛抗原的特性分析。
Zentralbl Veterinarmed B. 1991 Nov;38(9):689-700. doi: 10.1111/j.1439-0450.1991.tb00930.x.
5
New fimbrial antigen F165 from Escherichia coli serogroup O115 strains isolated from piglets with diarrhea.从患有腹泻的仔猪中分离出的大肠杆菌O115血清型菌株的新型菌毛抗原F165。
Infect Immun. 1986 Jan;51(1):10-5. doi: 10.1128/iai.51.1.10-15.1986.
6
Monoclonal antibody enzyme-linked immunosorbent assay for identification of K99-positive Escherichia coli isolates from calves.用于鉴定犊牛源K99阳性大肠杆菌分离株的单克隆抗体酶联免疫吸附测定法
J Clin Microbiol. 1984 Apr;19(4):498-501. doi: 10.1128/jcm.19.4.498-501.1984.
7
A new fimbrial antigen on Escherichia coli strains isolated from zebu (Bos indicus) calves with diarrhoea in Brazil.从巴西患腹泻的瘤牛（印度牛）犊中分离出的大肠杆菌菌株上的一种新的菌毛抗原。
Vet Rec. 1988 Jul 16;123(3):80-1. doi: 10.1136/vr.123.3.80-a.
8
Relative cell surface hydrophobicity of Escherichia coli strains with various recognized fimbrial antigens and without recognized fimbriae.具有各种已识别菌毛抗原和无已识别菌毛的大肠杆菌菌株的相对细胞表面疏水性
Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Aug;269(2):218-36. doi: 10.1016/s0176-6724(88)80100-7.
9
Colonization antigens and haemagglutination patterns of human Escherichia coli.人源大肠杆菌的定植抗原和血凝模式
Eur J Clin Microbiol. 1985 Jun;4(3):316-26. doi: 10.1007/BF02013660.
10
Detection of K88 and K99 fimbrial antigens on Escherichia coli by coagglutination.用协同凝集试验检测大肠杆菌上的K88和K99菌毛抗原。
Aust Vet J. 1987 Aug;64(8):239-40. doi: 10.1111/j.1751-0813.1987.tb09691.x.

引用本文的文献

1
Reduction of enterotoxin induced fluid accumulation in ileal loops of neonatal calves with anti-F5 fimbriae recombinant antibody.用抗F5菌毛重组抗体减少新生犊牛回肠袢中肠毒素诱导的液体蓄积。
Vet Res Commun. 2015 Dec;39(4):229-36. doi: 10.1007/s11259-015-9646-1. Epub 2015 Oct 31.
2
A single-chain fragment variable recombinant antibody against F5 fimbria of enterotoxigenic Escherichia coli inhibits agglutination of horse red blood cells induced by F5 protein.一种针对产肠毒素大肠杆菌F5菌毛的单链可变区片段重组抗体可抑制F5蛋白诱导的马红细胞凝集。
Vet Res Commun. 2005 Aug;29(6):463-76. doi: 10.1007/s11259-005-1432-z.

本文引用的文献

1
Mannose-resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis.甘露糖抗性血凝和P抗原识别是引起原发性肾盂肾炎的大肠杆菌的特征。
Lancet. 1981;2(8260-61):1366-9. doi: 10.1016/s0140-6736(81)92796-3.
2
Monoclonal antibody enzyme-linked immunosorbent assay for identification of K99-positive Escherichia coli isolates from calves.用于鉴定犊牛源K99阳性大肠杆菌分离株的单克隆抗体酶联免疫吸附测定法
J Clin Microbiol. 1984 Apr;19(4):498-501. doi: 10.1128/jcm.19.4.498-501.1984.
3
Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains.
非侵袭性产肠毒素大肠杆菌菌株的宿主特异性菌毛黏附素。
Microbiol Rev. 1982 Jun;46(2):129-61. doi: 10.1128/mr.46.2.129-161.1982.
4
The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in gram negative bacteria.细菌菌毛的结构、功能、合成及基因控制以及革兰氏阴性菌中DNA和RNA转运的分子模型。
Trans N Y Acad Sci. 1965 Jun;27(8):1003-54. doi: 10.1111/j.2164-0947.1965.tb02342.x.
5
Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent.引起腹泻的大肠杆菌：产肠毒素型、致病性、侵袭性、出血性和黏附性。
J Infect Dis. 1987 Mar;155(3):377-89. doi: 10.1093/infdis/155.3.377.
6
Monoclonal antibody passive hemagglutination and capture enzyme-linked immunosorbent assays for direct detection and quantitation of F41 and K99 fimbrial antigens in enterotoxigenic Escherichia coli.用于直接检测和定量产肠毒素大肠杆菌中F41和K99菌毛抗原的单克隆抗体被动血凝试验和捕获酶联免疫吸附测定
J Clin Microbiol. 1987 Feb;25(2):278-84. doi: 10.1128/jcm.25.2.278-284.1987.
7
Homogeneous distribution of parainfluenza virus glycoproteins demonstrated by immunogold-labelling and light staining with uranyl acetate in electron microscopy.免疫金标记和醋酸铀电子显微镜轻染色显示副流感病毒糖蛋白的均匀分布。
J Virol Methods. 1987 Mar;15(4):273-7. doi: 10.1016/0166-0934(87)90149-2.
8
Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor.从腹泻成人中分离出的产肠毒素大肠杆菌对人 A 型红细胞的血凝作用：与定植因子的相关性。
Infect Immun. 1977 Nov;18(2):330-7. doi: 10.1128/iai.18.2.330-337.1977.
9
Improved minca medium for the detection of K99 antigen in calf enterotoxigenic strains of Escherichia coli.用于检测犊牛产肠毒素大肠杆菌菌株中K99抗原的改良明卡培养基。
Infect Immun. 1977 Feb;15(2):676-8. doi: 10.1128/iai.15.2.676-678.1977.