Luo Gang, De Francisci Davide, Kougias Panagiotis G, Laura Treu, Zhu Xinyu, Angelidaki Irini
Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, 200433 Shanghai, China.
Department of Environmental Engineering, Technical University of Denmark, DK-2800 Kgs Lyngby Copenhagen, Denmark.
Biotechnol Biofuels. 2015 Jan 22;8:3. doi: 10.1186/s13068-014-0182-y. eCollection 2015.
The microbial community in a biogas reactor greatly influences the process performance. However, only the effects of deterministic factors (such as temperature and hydraulic retention time (HRT)) on the microbial community and performance have been investigated in biogas reactors. Little is known about the manner in which stochastic factors (for example, stochastic birth, death, colonization, and extinction) and disturbance affect the stable-state microbial community and reactor performances.
In the present study, three replicate biogas reactors treating cattle manure were run to examine the role of stochastic factors and disturbance in shaping microbial communities. In the triplicate biogas reactors with the same inoculum and operational conditions, similar process performances and microbial community profiles were observed under steady-state conditions. This indicated that stochastic factors had a minor role in shaping the profile of the microbial community composition and activity in biogas reactors. On the contrary, temperature disturbance was found to play an important role in the microbial community composition as well as process performance for biogas reactors. Although three different temperature disturbances were applied to each biogas reactor, the increased methane yields (around 10% higher) and decreased volatile fatty acids (VFAs) concentrations at steady state were found in all three reactors after the temperature disturbances. After the temperature disturbance, the biogas reactors were brought back to the original operational conditions; however, new steady-state microbial community profiles were observed in all the biogas reactors.
The present study demonstrated that temperature disturbance, but not stochastic factors, played an important role in shaping the profile of the microbial community composition and activity in biogas reactors. New steady-state microbial community profiles and reactor performances were observed in all the biogas reactors after the temperature disturbance.
沼气反应器中的微生物群落对工艺性能有很大影响。然而，在沼气反应器中，仅研究了确定性因素（如温度和水力停留时间（HRT））对微生物群落和性能的影响。对于随机因素（例如随机出生、死亡、定殖和灭绝）以及干扰如何影响稳态微生物群落和反应器性能知之甚少。
在本研究中，运行了三个处理牛粪的重复沼气反应器，以研究随机因素和干扰在塑造微生物群落中的作用。在接种物和操作条件相同的三个重复沼气反应器中，在稳态条件下观察到了相似的工艺性能和微生物群落概况。这表明随机因素在塑造沼气反应器中微生物群落组成和活性的概况方面作用较小。相反，发现温度干扰在沼气反应器的微生物群落组成以及工艺性能方面起着重要作用。尽管对每个沼气反应器施加了三种不同的温度干扰，但在温度干扰后，所有三个反应器在稳态下均发现甲烷产量增加（高出约10%）且挥发性脂肪酸（VFA）浓度降低。温度干扰后，将沼气反应器恢复到原始操作条件；然而，在所有沼气反应器中都观察到了新的稳态微生物群落概况。
本研究表明，温度干扰而非随机因素在塑造沼气反应器中微生物群落组成和活性的概况方面起着重要作用。温度干扰后，在所有沼气反应器中都观察到了新的稳态微生物群落概况和反应器性能。
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