Xu Leyuan, Sheybani Natasha, Yeudall W Andrew, Yang Hu
Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, Virginia 23284.
Philips Institute of Oral and Craniofacial Molecular Biology, Virginia Commonwealth University, Richmond, Virginia 23298 ; Massey Cancer Center; Virginia Commonwealth University, Richmond, Virginia 23298.
Biomater Sci. 2015 Feb;3(2):250-5. doi: 10.1039/C4BM00245H.
Free-radical photopolymerization initiated by photoinitiators is an important method to make tissue engineering scaffolds. To advance understanding of photoinitiator cytocompatibility, we examined three photoinitiators including 2,2-dimethoxy-2-phenylacetophenone (DMPA), Irgacure 2959 (I-2959), and eosin Y photoinitiating system (EY) in terms of their effects on viability of HN4 cells and expression levels of intracellular AKT and its phosphorylated form p-AKT. Our results show that the photoinitiators and their UV-exposed counterparts affect intracellular AKT signaling, which can be used in conjunction with cell viability for cytocompatibility assessment of photoinitiators.
由光引发剂引发的自由基光聚合是制备组织工程支架的重要方法。为了深入了解光引发剂的细胞相容性，我们研究了三种光引发剂，包括2,2-二甲氧基-2-苯基苯乙酮（DMPA）、Irgacure 2959（I-2959）和曙红Y光引发体系（EY），考察了它们对HN4细胞活力以及细胞内AKT及其磷酸化形式p-AKT表达水平的影响。我们的结果表明，光引发剂及其紫外线照射后的对应物会影响细胞内AKT信号传导，这可与细胞活力结合用于光引发剂的细胞相容性评估。
