Perez-Oso E, Lopez-Ruiz M P, Gonzalez-Guijarro L, Arilla E
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Alcalá de Henares, Madrid, Spain.
Life Sci. 1989;45(25):2451-8. doi: 10.1016/0024-3205(89)90010-6.
Rats were kindled by intraperitoneal injection of pentylenetetrazol (PTZ) (30 mg/Kg) every 48 h. Once kindled, some of the animals received a single injection of cysteamine (200 mg/Kg). Somatostatin-like immunoreactivity (SLI) and 125 I-Tyr11-somatostatin binding were measured in the frontoparietal cortex and hippocampus of the two experimental groups and the control rats. After PTZ kindling the following was observed: 1) SLI content was increased in the two areas; 2) Somatostatin receptor affinity decreased in the frontoparietal cortex and was unaltered in the hippocampus; 3) The number of somatostatin receptors decreased in the hippocampus and was unaltered in the frontoparietal cortex. Cysteamine, an agent which depletes brain somatostatin and suppresses kindled seizures in PTZ-treated rats, reversed the altered SLI levels and binding in these rats.
每隔48小时给大鼠腹腔注射戊四氮（PTZ，30毫克/千克）以诱导惊厥。一旦诱导成功，部分动物接受一次半胱胺注射（200毫克/千克）。在两个实验组和对照大鼠的额顶叶皮质和海马中测量生长抑素样免疫反应性（SLI）和¹²⁵I-酪氨酸¹¹-生长抑素结合情况。在PTZ诱导惊厥后观察到以下情况：1）两个区域的SLI含量均增加；2）额顶叶皮质中生长抑素受体亲和力降低，海马中未改变；3）海马中生长抑素受体数量减少，额顶叶皮质中未改变。半胱胺是一种可耗尽脑内生长抑素并抑制PTZ处理大鼠惊厥发作的药物，它可逆转这些大鼠中改变的SLI水平和结合情况。
