Kobayashi S, Kainuma K, Suzuki S
Carbohydr Res. 1978 Mar;61:229-38. doi: 10.1016/s0008-6215(00)84484-5.
Bacillus macerans cycloamylose (cyclodextrin) glucanotransferase (EC 2.4.1.19) was purified by the technique of starch adsorption and DEAE-cellulose column chromatography, and then crystallized from an ammonium sulfate solution containing mM calcium chloride. The crystals of the enzyme were rod-shaped and showed a single band by disc-gel electrophorsis. The purified enzyme was dissociated into two subunits by sodium dodecyl sulfate-disc electrophoresis. The subunits had no enzyme activity. Details of each purification step and some properties of the enzyme are described in this paper.
浸麻芽孢杆菌环糊精（环淀粉酶）葡聚糖转移酶（EC 2.4.1.19）通过淀粉吸附和DEAE-纤维素柱色谱技术进行纯化，然后从含有mM氯化钙的硫酸铵溶液中结晶。该酶的晶体呈棒状，在圆盘凝胶电泳中显示为一条带。通过十二烷基硫酸钠-圆盘电泳，纯化后的酶解离为两个亚基。这些亚基没有酶活性。本文描述了每个纯化步骤的细节以及该酶的一些性质。
