• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

浸麻芽孢杆菌环糊精葡聚糖转移酶基因的分子克隆、DNA核苷酸测序及在枯草芽孢杆菌细胞中的表达

Molecular cloning, DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene.

作者信息

Takano T, Fukuda M, Monma M, Kobayashi S, Kainuma K, Yamane K

出版信息

J Bacteriol. 1986 Jun;166(3):1118-22. doi: 10.1128/jb.166.3.1118-1122.1986.

DOI:10.1128/jb.166.3.1118-1122.1986
PMID:3011735
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215240/
Abstract

The gene for cyclodextrin glucanotransferase from Bacillus macerans was cloned in an Escherichia coli bacteriophage, lambda D69, and was recloned in a Bacillus subtilis plasmid, pUB110. Starting from an ATG initiation codon, a unique reading frame was shown to extend for 2,142 base pairs (714 amino acids). The nucleotide sequence revealed that the enzyme is composed of two identical subunits.

摘要

来自浸麻芽孢杆菌的环糊精葡糖基转移酶基因被克隆到大肠杆菌噬菌体λ D69中，并再次克隆到枯草芽孢杆菌质粒pUB110中。从ATG起始密码子开始，一个独特的阅读框显示延伸2142个碱基对（714个氨基酸）。核苷酸序列表明该酶由两个相同的亚基组成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86f7/215240/b296a9efd2bf/jbacter00211-0434-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86f7/215240/f893b8988ed2/jbacter00211-0434-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86f7/215240/b296a9efd2bf/jbacter00211-0434-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86f7/215240/f893b8988ed2/jbacter00211-0434-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86f7/215240/b296a9efd2bf/jbacter00211-0434-b.jpg

相似文献

1
Molecular cloning, DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene.浸麻芽孢杆菌环糊精葡聚糖转移酶基因的分子克隆、DNA核苷酸测序及在枯草芽孢杆菌细胞中的表达
J Bacteriol. 1986 Jun;166(3):1118-22. doi: 10.1128/jb.166.3.1118-1122.1986.
2
Molecular cloning and nucleotide sequence of the cyclomaltodextrin glucanotransferase gene from the alkalophilic Bacillus sp. strain no. 38-2.嗜碱芽孢杆菌属38-2号菌株环糊精葡萄糖基转移酶基因的分子克隆及核苷酸序列
J Gen Microbiol. 1988 Jan;134(1):97-105. doi: 10.1099/00221287-134-1-97.
3
Nucleotide sequence of the raw-starch-digesting amylase gene from Bacillus sp. B1018 and its strong homology to the cyclodextrin glucanotransferase genes.芽孢杆菌属B1018的生淀粉消化淀粉酶基因的核苷酸序列及其与环糊精葡聚糖转移酶基因的高度同源性。
Biochem Biophys Res Commun. 1990 Jan 30;166(2):630-6. doi: 10.1016/0006-291x(90)90855-h.
4
Cyclization characteristics of cyclodextrin glucanotransferase are conferred by the NH2-terminal region of the enzyme.环糊精葡糖基转移酶的环化特性由该酶的氨基末端区域赋予。
Appl Environ Microbiol. 1992 Dec;58(12):4016-25. doi: 10.1128/aem.58.12.4016-4025.1992.
5
Cloning and sequencing of a cyclodextrin glucanotransferase gene from Bacillus ohbensis and its expression in Escherichia coli.
Appl Microbiol Biotechnol. 1991 Aug;35(5):600-5. doi: 10.1007/BF00169623.
6
Alpha-amylase genes (amyR2 and amyE+) from an alpha-amylase-hyperproducing Bacillus subtilis strain: molecular cloning and nucleotide sequences.来自一株α-淀粉酶高产枯草芽孢杆菌菌株的α-淀粉酶基因（amyR2和amyE+）：分子克隆及核苷酸序列
J Bacteriol. 1983 Oct;156(1):327-37. doi: 10.1128/jb.156.1.327-337.1983.
7
Cloning of the cyclodextrin glucanotransferase gene from alkalophilic Bacillus sp. A2-5a and analysis of the raw starch-binding domain.嗜碱芽孢杆菌A2-5a环糊精葡萄糖基转移酶基因的克隆及生淀粉结合域分析
Appl Microbiol Biotechnol. 2000 Apr;53(4):430-4. doi: 10.1007/s002530051637.
8
Molecular cloning, nucleotide sequence and expression in Escherichia coli of the beta-cyclodextrin glycosyltransferase gene from Bacillus circulans strain no. 8.环状芽孢杆菌8号菌株β-环糊精糖基转移酶基因的分子克隆、核苷酸序列及在大肠杆菌中的表达
Appl Microbiol Biotechnol. 1990 Aug;33(5):542-6. doi: 10.1007/BF00172548.
9
Expression of the beta-cyclodextrin glucanotransferase gene of an alkalophilic Bacillus sp. #1011 in Escherichia coli cells and characterization of the synthesized enzyme.嗜碱芽孢杆菌#1011的β-环糊精葡聚糖转移酶基因在大肠杆菌细胞中的表达及合成酶的特性研究
Agric Biol Chem. 1990 Mar;54(3):641-8.
10
Transcriptional regulation of the Bacillus ohbensis cyclodextrin glucanotransferase gene in B. subtilis.枯草芽孢杆菌中奥氏芽孢杆菌环糊精葡聚糖转移酶基因的转录调控
Biosci Biotechnol Biochem. 1999 Nov;63(11):1902-9. doi: 10.1271/bbb.63.1902.

引用本文的文献

1
Stepwise error-prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilic Bacillus sp.逐步易错PCR和DNA改组改变了嗜碱芽孢杆菌环糊精葡糖基转移酶的pH活性范围和产物特异性。
FEBS Open Bio. 2015 Jun 11;5:528-34. doi: 10.1016/j.fob.2015.06.002. eCollection 2015.
2
Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications.环糊精葡萄糖基转移酶的工程改造及其在生物技术应用中的影响。
Appl Microbiol Biotechnol. 2010 Jan;85(4):823-35. doi: 10.1007/s00253-009-2221-3. Epub 2009 Sep 18.
3
Cloning, extracellular expression and characterization of a predominant beta-CGTase from Bacillus sp. G1 in E. coli.

本文引用的文献

1
Detection of sugars on paper chromatograms.纸色谱上糖的检测
Nature. 1950 Sep 9;166(4219):444-5. doi: 10.1038/166444b0.
2
Amino-terminal analysis of polypeptide using dimethylaminoazobenzene isothiocyanate.使用二甲基氨基偶氮苯异硫氰酸酯对多肽进行氨基末端分析。
Anal Biochem. 1980 Mar 1;102(2):384-92. doi: 10.1016/0003-2697(80)90172-4.
3
Alpha-amylase genes (amyR2 and amyE+) from an alpha-amylase-hyperproducing Bacillus subtilis strain: molecular cloning and nucleotide sequences.来自一株α-淀粉酶高产枯草芽孢杆菌菌株的α-淀粉酶基因（amyR2和amyE+）：分子克隆及核苷酸序列
芽孢杆菌属G1中一种主要的β-环糊精葡萄糖基转移酶在大肠杆菌中的克隆、胞外表达及特性分析
J Ind Microbiol Biotechnol. 2008 Dec;35(12):1705-14. doi: 10.1007/s10295-008-0462-2. Epub 2008 Aug 26.
4
Protein secretion in Bacillus species.芽孢杆菌属中的蛋白质分泌
Microbiol Rev. 1993 Mar;57(1):109-37. doi: 10.1128/mr.57.1.109-137.1993.
5
Cloning of the beta-amylase gene from Bacillus cereus and characteristics of the primary structure of the enzyme.蜡样芽孢杆菌β-淀粉酶基因的克隆及其酶一级结构的特性
Appl Environ Microbiol. 1993 Feb;59(2):623-7. doi: 10.1128/aem.59.2.623-627.1993.
6
Molecular structure of the SWA2 gene encoding an AMY1-related alpha-amylase from Schwanniomyces occidentalis.编码来自西方许旺酵母的一种与AMY1相关的α-淀粉酶的SWA2基因的分子结构。
Curr Genet. 1993 Jul-Aug;24(1-2):75-83. doi: 10.1007/BF00324668.
7
Cloning and sequence analysis of the cyclomaltodextrinase gene from Bacillus sphaericus and expression in Escherichia coli cells.球形芽孢杆菌环糊精酶基因的克隆、序列分析及其在大肠杆菌中的表达
Appl Microbiol Biotechnol. 1993 May;39(2):197-203. doi: 10.1007/BF00228606.
8
Nucleotide sequence of the beta-cyclodextrin glucanotransferase gene of alkalophilic Bacillus sp. strain 1011 and similarity of its amino acid sequence to those of alpha-amylases.嗜碱芽孢杆菌菌株1011的β-环糊精葡聚糖转移酶基因的核苷酸序列及其氨基酸序列与α-淀粉酶氨基酸序列的相似性
J Bacteriol. 1987 Sep;169(9):4399-402. doi: 10.1128/jb.169.9.4399-4402.1987.
9
Characterization of the genes for the hexagonally arranged surface layer proteins in protein-producing Bacillus brevis 47: complete nucleotide sequence of the middle wall protein gene.产蛋白短短芽孢杆菌47中六边形排列的表层蛋白基因的特性：中壁蛋白基因的完整核苷酸序列
J Bacteriol. 1988 Feb;170(2):935-45. doi: 10.1128/jb.170.2.935-945.1988.
10
A super-secondary structure predicted to be common to several alpha-1,4-D-glucan-cleaving enzymes.一种预测为几种α-1,4-D-葡聚糖裂解酶共有的超二级结构。
Biochem J. 1989 Apr 1;259(1):145-52. doi: 10.1042/bj2590145.
J Bacteriol. 1983 Oct;156(1):327-37. doi: 10.1128/jb.156.1.327-337.1983.
4
New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
5
A bacteriophage lambda vector for cloning with BamHI and Sau3A.一种用于用BamHI和Sau3A进行克隆的λ噬菌体载体。
Gene. 1982 Dec;20(3):317-22. doi: 10.1016/0378-1119(82)90200-1.
6
Purification and properties of the amylase of Bacillus macerans.浸麻芽孢杆菌淀粉酶的纯化及性质
Biochemistry. 1968 Jan;7(1):114-20. doi: 10.1021/bi00841a015.
7
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.聚乙二醇存在下噬菌体的快速沉降及其在大规模病毒纯化中的应用
Virology. 1970 Mar;40(3):734-44. doi: 10.1016/0042-6822(70)90218-7.
8
A method for the recovery of DNA from agarose gels.一种从琼脂糖凝胶中回收DNA的方法。
Nucleic Acids Res. 1978 Jul;5(7):2321-32. doi: 10.1093/nar/5.7.2321.
9
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.一种用于筛选重组质粒DNA的快速碱性提取方法。
Nucleic Acids Res. 1979 Nov 24;7(6):1513-23. doi: 10.1093/nar/7.6.1513.
10
DNA sequencing with chain-terminating inhibitors.使用链终止抑制剂的DNA测序。
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7. doi: 10.1073/pnas.74.12.5463.