浸麻芽孢杆菌环糊精葡聚糖转移酶基因的分子克隆、DNA核苷酸测序及在枯草芽孢杆菌细胞中的表达
Molecular cloning, DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene.
作者信息
Takano T, Fukuda M, Monma M, Kobayashi S, Kainuma K, Yamane K
出版信息
J Bacteriol. 1986 Jun;166(3):1118-22. doi: 10.1128/jb.166.3.1118-1122.1986.
Abstract
The gene for cyclodextrin glucanotransferase from Bacillus macerans was cloned in an Escherichia coli bacteriophage, lambda D69, and was recloned in a Bacillus subtilis plasmid, pUB110. Starting from an ATG initiation codon, a unique reading frame was shown to extend for 2,142 base pairs (714 amino acids). The nucleotide sequence revealed that the enzyme is composed of two identical subunits.
摘要
来自浸麻芽孢杆菌的环糊精葡糖基转移酶基因被克隆到大肠杆菌噬菌体λ D69中，并再次克隆到枯草芽孢杆菌质粒pUB110中。从ATG起始密码子开始，一个独特的阅读框显示延伸2142个碱基对（714个氨基酸）。核苷酸序列表明该酶由两个相同的亚基组成。
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