Zhang De-Chao, Wu Jiang, Neuner Kathrin, Yao Jianting, Margesin Rosa
Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, PR China.
College of Agronomy & Resources and Environment, Tianjin Agricultural University, 300384 Tianjin, PR China.
Int J Syst Evol Microbiol. 2015 May;65(Pt 5):1556-1560. doi: 10.1099/ijs.0.000134. Epub 2015 Feb 23.
A Gram-reaction-negative, rod-shaped, yellow-pigmented, motile by gliding bacterial strain, designated RU-4-M-4(T), was isolated from intertidal sediment of Sakhalin Island in Russia. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RU-4-M-4(T) was related to the genus Algibacter and had highest 16S rRNA gene sequence similarity with Algibacter pectinivorans KACC 14153(T) (97.2%). The major cellular fatty acids were iso-C15 : 0 3-OH, C15: 0 and iso-C15 : 1 G. The predominant menaquinone was MK-6. The polar lipid profile contained phosphatidylethanolamine, three unidentified aminolipids and two unidentified lipids. The genomic DNA G+C content of strain RU-4-M-4(T) was 36.4 mol%. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain RU-4-M-4(T) is a representative of a novel species of the genus Algibacter , for which we propose the name Algibacter amylolyticus sp. nov. (type strain RU-4-M-4(T) =LMG 28383(T) =DSM 29199(T)).
从俄罗斯萨哈林岛潮间带沉积物中分离出一株革兰氏阴性、杆状、产黄色色素、靠滑行运动的细菌菌株，命名为RU-4-M-4(T)。基于16S rRNA基因序列的系统发育分析表明，菌株RU-4-M-4(T)与Algibacter属相关，与果胶食Algibacter pectinivorans KACC 14153(T)的16S rRNA基因序列相似性最高（97.2%）。主要细胞脂肪酸为异-C15 : 0 3-OH、C15: 0和异-C15 : 1 G。主要的甲基萘醌是MK-6。极性脂质谱包含磷脂酰乙醇胺、三种未鉴定的氨基脂质和两种未鉴定的脂质。菌株RU-4-M-4(T)的基因组DNA G+C含量为36.4 mol%。表型、系统发育和DNA-DNA相关性研究的综合数据表明，菌株RU-4-M-4(T)是Algibacter属一个新物种的代表，我们将其命名为解淀粉Algibacter amylolyticus sp. nov.（模式菌株RU-4-M-4(T) =LMG 28383(T) =DSM 29199(T)）。
