Marine College, Shandong University, Weihai 264209, PR China.
State key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, PR China.
Int J Syst Evol Microbiol. 2020 Sep;70(9):5048-5053. doi: 10.1099/ijsem.0.004375.
A Gram-stain-negative, non-flagellated bacterium, designated ZY111, was isolated from the surface of a marine red alga, which was collected from the coast in Weihai, Shandong Province, PR China. Strain ZY111 exhibited growth at 4-37 °C (optimum, 25-28 °C) in the presence of 0-8.0 % (w/v) NaCl (optimum, 2.0-4.0% NaCl) and at pH 6.5-9.5 (optimum, pH 7.0-8.0). The 16S rRNA gene sequence analysis revealed that strain ZY111 belonged to the genus , with DSM 29199 as its closest relative (97.7 % similarity). The averagenucleotide identity value of strain ZY111 with DSM 29199 was 79.03 %. The digitalDNA-DNA hybridization value of strain ZY111 with DSM 29199 was 22.40 %. The dominant fatty acids were iso-C, iso-C G, iso-C 3-OH and iso-C 3-OH. The sole respiratory quinone was determined to be menaquinone-6. The polar lipid profile of strain ZY111 consisted of phosphatidylethanolamine, two unidentified aminolipids and three unidentified lipids. The G+C content was 31.9 mol%. The phenotypic, chemotaxonomic and phylogenetic data clearly showed that strain ZY111 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ZY111 (=KCTC 62373=MCCC 1H00295).
一株革兰氏阴性、无鞭毛的细菌，被命名为 ZY111，从中国山东省威海市海岸采集的海洋红藻表面分离得到。菌株 ZY111 在 4-37°C（最佳温度为 25-28°C）、0-8.0%（w/v）NaCl（最佳盐度为 2.0-4.0%NaCl）和 pH 6.5-9.5（最佳 pH 值为 7.0-8.0）的条件下生长。16S rRNA 基因序列分析表明，菌株 ZY111 属于 属，与 DSM 29199 最为密切（相似度为 97.7%）。菌株 ZY111 与 DSM 29199 的平均核苷酸同一性值为 79.03%。菌株 ZY111 与 DSM 29199 的 DNA-DNA 杂交值为 22.40%。菌株 ZY111 的主要脂肪酸为 iso-C、iso-C G、iso-C 3-OH 和 iso-C 3-OH。唯一的呼吸醌为menaquinone-6。菌株 ZY111 的极性脂谱由磷脂酰乙醇胺、两种未鉴定的氨基脂和三种未鉴定的脂组成。G+C 含量为 31.9 mol%。表型、化学分类和系统发育数据清楚地表明，菌株 ZY111 代表了 属的一个新种，因此提议将其命名为 sp. nov.。其模式菌株为 ZY111（=KCTC 62373=MCCC 1H00295）。
