Department of Bioengineering, College of Marine Sciences and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
University of Chinese Academy of Sciences, Beijing 100049, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2907-2911. doi: 10.1099/ijsem.0.004119. Epub 2020 Apr 3.
The Gram-stain-negative, rod-shaped, yellow-pigmented and facultative anaerobic bacterial strain, designated H164, was isolated from seawater collected from the Caroline Seamounts in the Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain H164 was related to the genus and had highest 16S rRNA gene sequence similarity to WS-MY22 (97.4 %). The major cellular fatty acids were iso-C, anteiso-C, iso-C G, iso-C 3-OH and iso-C 3-OH. The predominant menaquinone was MK-6. The polar lipid profile contained phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The genomic DNA G+C content of strain H164 was 33.2 mol%. The values of DNA-DNA hybridization (DDH) and average nucleotide identity (ANI) between strain H164 and KCTC 32381 were 26.10 and 81.88 %. The DDH and ANI values between strain H164 and DSM 15365 were 25.40 and 81.79 %. Combined data from phenotypic, phylogenetic, DDH and ANI analyses demonstrated that strain H164 represents a novel species of the genus , for which we propose the name sp. nov. (type strain H164=KCTC 72432=CGMCC 1.17117).
革兰氏阴性、杆状、黄色素、兼性厌氧的细菌菌株 H164 是从太平洋卡罗琳海山采集的海水中分离出来的。基于 16S rRNA 基因序列的系统发育分析表明，菌株 H164 与属有关，与 WS-MY22（97.4%）的 16S rRNA 基因序列相似度最高。主要细胞脂肪酸为 iso-C、anteiso-C、iso-C G、iso-C 3-OH 和 iso-C 3-OH。主要的menaquinone 是 MK-6。极性脂谱包含磷脂酰乙醇胺、一种未鉴定的氨基脂和两种未鉴定的脂。菌株 H164 的基因组 DNA G+C 含量为 33.2mol%。菌株 H164 与 KCTC 32381 之间的 DNA-DNA 杂交（DDH）和平均核苷酸同一性（ANI）值分别为 26.10%和 81.88%。菌株 H164 与 DSM 15365 之间的 DDH 和 ANI 值分别为 25.40%和 81.79%。表型、系统发育、DDH 和 ANI 分析的综合数据表明，菌株 H164 代表了属的一个新种，我们建议将其命名为 sp. nov.（模式菌株 H164=KCTC 72432=CGMCC 1.17117）。
