Bak Jin Seop
Department of Chemical and Biomolecular Engineering, Advanced Biomass R&D Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Biotechnol Rep (Amst). 2014 Jul 31;4:30-33. doi: 10.1016/j.btre.2014.07.006. eCollection 2014 Dec.
In order to overcome the limitation of commercial electron beam irradiation (EBI), lignocellulosic rice straw (RS) was pretreated using water soaking-based electron beam irradiation (WEBI). This environment-friendly pretreatment, without the formation (or release) of inhibitory compounds (especially hydroxymethylfurfural and furfural), significantly increased the enzymatic hydrolysis and fermentation yields of RS. Specifically, when water-soaked RS (solid:liquid ratio of 100%) was treated with WEBI doses of 1 MeV at 80 kGy, 0.12 mA, the glucose yield after 120 h of hydrolysis was 70.4% of the theoretical maximum. This value was predominantly higher than the 29.5% and 52.1% measured from untreated and EBI-treated RS, respectively. Furthermore, after simultaneous saccharification and fermentation for 48 h, the ethanol concentration, production yield, and productivity were 9.3 g/L, 57.0% of the theoretical maximum, and 0.19 g/L h, respectively. Finally, scanning electron microscopy images revealed that WEBI induced significant ultrastructural changes to the surface of lignocellulosic fibers.
为了克服商业电子束辐照（EBI）的局限性，采用基于水浸的电子束辐照（WEBI）对木质纤维素稻草（RS）进行预处理。这种环保型预处理不会形成（或释放）抑制性化合物（尤其是羟甲基糠醛和糠醛），显著提高了RS的酶水解和发酵产率。具体而言，当水浸RS（固液比为100%）用剂量为1 MeV、80 kGy、0.12 mA的WEBI处理时，水解120 h后的葡萄糖产率为理论最大值的70.4%。该值明显高于未处理和EBI处理的RS分别测得的29.5%和52.1%。此外，在同步糖化发酵48 h后，乙醇浓度、产率和生产率分别为9.3 g/L、理论最大值的57.0%和0.19 g/（L·h）。最后，扫描电子显微镜图像显示，WEBI引起了木质纤维素纤维表面显著的超微结构变化。
