Popović Višnja, Stojković Dejan, Nikolić Miloš, Heyerick Arne, Petrović Silvana, Soković Marina, Niketić Marjan
University of Belgrade - Faculty of Pharmacy, Department of Pharmacognosy, V. Stepe 450, 11221 Belgrade, Serbia.
Food Funct. 2015 Apr;6(4):1205-11. doi: 10.1039/c5fo00066a.
Antimicrobial properties of extracts of underground parts of three Laserpitium L. (Apiaceae) species, namely Laserpitium latifolium L., Laserpitium zernyi Hayek and Laserpitium ochridanum Micevski, were investigated. The investigated species are widely used as functional foods, as spices and for preparations in traditional medicine for treating complaints connected with infection and inflammation. Furthermore, antimicrobial and antibiofilm effects of laserpitine, the most abundant compound in the chloroform extract of L. latifolium, and guaianolide sesquiterpene lactones, such as, isomontanolide, montanolide and tarolide, principal components of the extracts of L. zernyi and L. ochridanum were assessed. The antimicrobial activity was tested using the microdilution method against five pathogenic bacteria and five fungi, as well as in the microplate biofilm assay on two Candida clinical isolates (C. albicans and C. krusei). Among the extracts, L. latifolium showed the most prominent activity. Isolated metabolites exerted higher effects against fungal than against bacterial strains, isomontanolide being the most active. Interestingly, all constituents showed higher potential on inhibition of biofilm formation than fluconazole, a reference compound. Tested metabolites may be good novel agents with high antifungal and antibacterial potential that might find practical applications in food industry as food preservatives in order to retard the growth of food spoiling microbes, but only after detailed safety assessments.
对三种绵果芹属（伞形科）植物地下部分的提取物抗菌特性进行了研究，这三种植物分别是宽叶绵果芹、泽尔尼绵果芹和奥赫里德绵果芹。这些被研究的植物被广泛用作功能性食品、香料以及用于传统医学中治疗与感染和炎症相关的病症。此外，还评估了宽叶绵果芹氯仿提取物中含量最高的化合物绵果芹碱，以及泽尔尼绵果芹和奥赫里德绵果芹提取物的主要成分愈创木烷型倍半萜内酯（如异蒙坦诺内酯、蒙坦诺内酯和塔罗内酯）的抗菌和抗生物膜作用。采用微量稀释法对五种致病细菌和五种真菌进行抗菌活性测试，并在微孔板生物膜试验中对两种念珠菌临床分离株（白色念珠菌和克柔念珠菌）进行测试。在提取物中，宽叶绵果芹表现出最显著的活性。分离出的代谢产物对真菌的作用比对细菌菌株的作用更强，异蒙坦诺内酯活性最强。有趣的是，所有成分在抑制生物膜形成方面比参考化合物氟康唑具有更高的潜力。经过测试的代谢产物可能是具有高抗真菌和抗菌潜力的新型优良药剂，在经过详细的安全性评估后，有可能作为食品防腐剂在食品工业中找到实际应用，以抑制食品腐败微生物的生长。
