用于病毒繁殖的鸡肾细胞培养物的制备。
The preparation of chicken kidney cell cultures for virus propagation.
作者信息
Hennion Ruth M, Hill Gillian
机构信息
The Pirbright Institute, High Street, Compton, Newbury, Berkshire, RG20 7NN, UK,
出版信息
Methods Mol Biol. 2015;1282:57-62. doi: 10.1007/978-1-4939-2438-7_6.
Abstract
Chicken kidney (CK) cell cultures have historically proved useful for the assay of a number of viruses including coronaviruses. A technique for the preparation of such cell cultures, using a combination of manual and trypsin disaggregation of kidneys dissected from 2- to 3-week-old birds is described. This technique routinely gives high cell yield together with high viability and the resultant adherent primary cultures can be used for virus growth and plaque formation.
摘要
鸡肾（CK）细胞培养物历来被证明可用于多种病毒的检测，包括冠状病毒。本文描述了一种制备此类细胞培养物的技术，该技术结合了手动操作以及使用胰蛋白酶对从2至3周龄鸡身上取出的肾脏进行解离。该技术通常能产生高细胞产量和高活力，所得的贴壁原代培养物可用于病毒生长和蚀斑形成。
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