Kingsley D M, Jenkins N A, Copeland N G
Mammalian Genetics Laboratory, NCI-Frederick Cancer Research Facility, Maryland 21701.
Genetics. 1989 Sep;123(1):165-72. doi: 10.1093/genetics/123.1.165.
A 64-centiMorgan linkage map of mouse chromosome 9 was developed using cloned DNA markers and an interspecific backcross between Mus spretus and the C57BL/6J inbred strain. This map was compared to conventional genetic maps using six markers previously localized in laboratory mouse strains. These markers included thymus cell antigen-1, cytochrome P450-3, dilute, transferrin, cholecystokinin, and the G-protein alpha inhibitory subunit. No evidence was seen for segregation distortion, chromosome rearrangements, or altered genetic distances in the results from interspecific backcross mapping. Regional map locations were determined for four genes that were previously assigned to chromosome 9 using somatic cell hybrids. These genes were glutathione S-transferase Ya subunit (Gsta), the T3 gamma subunit, the low density lipoprotein receptor, and the Ets-1 oncogene. The map locations for these genes establish new regions of synteny between mouse chromosome 9 and human chromosomes 6, 11, and 19. In addition, the close linkage detected between the dilute and Gsta loci suggests that the Gsta locus may be part of the dilute/short ear complex, one of the most extensively studied genetic regions of the mouse.
利用克隆的DNA标记以及小家鼠（Mus spretus）与近交系C57BL/6J之间的种间回交，构建了小鼠9号染色体的一个64厘摩的连锁图谱。使用先前定位在实验室小鼠品系中的6个标记，将该图谱与传统遗传图谱进行了比较。这些标记包括胸腺细胞抗原-1、细胞色素P450-3、稀释基因、转铁蛋白、胆囊收缩素和G蛋白α抑制亚基。在种间回交定位的结果中，未发现分离畸变、染色体重排或遗传距离改变的证据。利用体细胞杂种，确定了先前定位到9号染色体上的4个基因的区域图谱位置。这些基因是谷胱甘肽S-转移酶Ya亚基（Gsta）、T3γ亚基、低密度脂蛋白受体和Ets-1癌基因。这些基因的图谱位置确定了小鼠9号染色体与人类6号、11号和19号染色体之间新的同线性区域。此外，在稀释基因和Gsta位点之间检测到的紧密连锁表明，Gsta位点可能是稀释/短耳复合体的一部分，该复合体是小鼠研究最为广泛的遗传区域之一。
