Tran H H, Ehsani S, Shibayama K, Matsui M, Suzuki S, Nguyen M B, Tran D N, Tran V P, Tran D L, Nguyen H T, Dang D A, Trinh H S, Nguyen T H, Wertheim H F L
National Institute of Hygiene and Epidemiology, Yersin Street 1, Hanoi, Vietnam,
Eur J Clin Microbiol Infect Dis. 2015 Jun;34(6):1247-54. doi: 10.1007/s10096-015-2345-6. Epub 2015 Mar 3.
This study sought to monitor the presence of carbapenem-resistant Enterobacteriaceae (CRE) and the proportion New Delhi metallo-beta-lactamase 1 (NDM-1)-producing bacteria between August 2010 and December 2012 in a surgical hospital in Vietnam. We identified 47 CRE strains from a total of 4,096 Enterobacteriaceae isolates (1.1 %) that were NDM-1-positive from 45 patients admitted to 11 different departments, with the majority being from the urology department. The NDM-1 gene was found in seven different species. Genotyping revealed limited clonality of NDM-1-positive isolates. Most of the isolates carried the NDM-1 gene on a plasmid and 17.8 % (8/45) of those were readily transferable. We found five patients at admission and one patient at discharge with NDM-1-positive bacteria in their stool. From 200 screening environmental hospital samples, five were confirmed to be NDM-1-positive and included Acinetobacter species (n = 3) and Enterobacter aerogenes (n = 2). The results reveal that NDM-1-producing Enterobacteriaceae are commonly isolated in patients admitted to a Vietnamese surgical hospital and are also detected in the hospital environment.
本研究旨在监测2010年8月至2012年12月期间越南一家外科医院中耐碳青霉烯类肠杆菌科细菌（CRE）的存在情况以及产新德里金属β-内酰胺酶1（NDM-1）细菌的比例。我们从总共4096株肠杆菌科分离株中鉴定出47株CRE菌株（1.1%），这些菌株来自11个不同科室收治的45例患者，其中大多数来自泌尿外科。NDM-1基因在7种不同菌种中被发现。基因分型显示NDM-1阳性分离株的克隆性有限。大多数分离株的NDM-1基因位于质粒上，其中17.8%（8/45）易于转移。我们发现5例入院患者和1例出院患者粪便中存在NDM-1阳性细菌。在200份医院环境筛查样本中，有5份被确认为NDM-1阳性，包括不动杆菌属（n = 3）和产气肠杆菌（n = 2）。结果显示，产NDM-1的肠杆菌科细菌在越南一家外科医院收治的患者中普遍分离得到，并且在医院环境中也被检测到。
