Shibasaki Seiji, Ueda Mitsuyoshi
General Education Center; Graduate School of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe 650-8530, Japan. seiji(a)huhs.ac.jp
Biocontrol Sci. 2014;19(4):157-64. doi: 10.4265/bio.19.157.
Molecular display techniques using microbial cell surfaces have been widely developed in the past twenty years, and are useful tools as whole cell catalysts for various applications such as bioconversion, bioremediation, biosensing, and the screening system of protein libraries. Furthermore, different types of microbial cells among eukaryotic and prokaryotic strains have been investigated for their use in surface display technologies. Recently, several kinds of protein-displaying yeasts have been utilized as bioadsorbents in this platform technology. In particular, these trials have successfully expanded the possibility of applications to metal binding, affinity purification, and receptor-ligand interaction by using the yeast cell surface. In this mini review, we describe the general principles of molecular display technology using yeast cells and its applications, with a particular focus on bioadsorption.
在过去二十年中，利用微生物细胞表面的分子展示技术得到了广泛发展，并且作为全细胞催化剂，在生物转化、生物修复、生物传感以及蛋白质文库筛选系统等各种应用中都是有用的工具。此外，真核和原核菌株中的不同类型微生物细胞已被研究用于表面展示技术。最近，几种展示蛋白质的酵母已被用作该平台技术中的生物吸附剂。特别是，这些试验通过利用酵母细胞表面成功扩展了在金属结合、亲和纯化和受体 - 配体相互作用方面的应用可能性。在本综述中，我们描述了利用酵母细胞的分子展示技术的一般原理及其应用，特别关注生物吸附。
