酪蛋白激酶 2 负调控核心脱落酸信号模块中脱落酸激活的 SnRK2s。

Casein Kinase 2 Negatively Regulates Abscisic Acid-Activated SnRK2s in the Core Abscisic Acid-Signaling Module.

机构信息

Centre for Research in Agricultural Genomics, Parc de Recerca UAB, Edifici CRAG, Campus UAB, Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain.

出版信息

Mol Plant. 2015 May;8(5):709-21. doi: 10.1016/j.molp.2014.12.012. Epub 2015 Jan 13.

DOI:10.1016/j.molp.2014.12.012

PMID:25744360

Abstract

SnRK2 kinases, PP2C phosphatases and the PYR/PYL/RCAR receptors constitute the core abscisic acid (ABA) signaling module that is thought to contain all of the intrinsic properties to self-regulate the hormone signal output. Here we identify Casein Kinase (CK)2 as a novel negative regulator of SnRK2. CK2 phosphorylates a cluster of conserved serines at the ABA box of SnRK2, increasing its binding to PP2C and triggering protein degradation. Consequently, CK2 action has implications on SnRK2 protein levels, as well as kinase activity and its response to abiotic stimuli.

摘要

SnRK2 激酶、PP2C 磷酸酶和 PYR/PYL/RCAR 受体构成了核心脱落酸（ABA）信号模块，该模块被认为包含了自我调节激素信号输出的所有内在特性。在这里，我们鉴定出酪蛋白激酶（CK）2 是 SnRK2 的一种新型负调节剂。CK2 磷酸化 SnRK2 ABA 盒上的一组保守丝氨酸，增加其与 PP2C 的结合并触发蛋白降解。因此，CK2 的作用对 SnRK2 蛋白水平、激酶活性及其对非生物刺激的反应都有影响。