Le Flèche-Matéos Anne, Levast Marion, Lomprez Fabienne, Arnoux Yolande, Andonian Clément, Perraud Michel, Vincent Véronique, Ar Gouilh Meriadeg, Thiberge Jean-Michel, Vandenbogaert Mathias, Diancourt Laure, Caro Valérie, Burguière Ana Maria, Manuguerra Jean-Claude
Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, F-75724 Paris Cedex 15, France.
Laboratoire de bactériologie, Centre Hospitalier de Chambéry, F-73011 Chambéry Cedex, France.
Int J Syst Evol Microbiol. 2015 Jun;65(Pt 6):1812-1818. doi: 10.1099/ijs.0.000179. Epub 2015 Mar 6.
Parenteral nutrition bags for newborns were found contaminated by a previously undescribed member of the family Enterobacteriaceae. The six isolates studied by rrs gene (encoding 16S rRNA) sequence analysis and multi-locus sequence analysis (MLSA) formed a discrete branch close to the genera Ewingella, Rahnella, Yersinia,Hafnia and Serratia. Phenotypically, the new taxon was distinct from these five genera. The new taxon gave positive results in Voges-Proskauer, Simmons citrate and o-nitrophenyl-β-galactoside hydrolysis tests; fermented d-glucose, d-mannitol, l-rhamnose, melibiose, l-arabinose and d-xylose; hydrolysed aesculin; and did not ferment maltose, trehalose, raffinose, d-sorbitol, sucrose or cellobiose. Tests for motility, gas production, urease, gelatinase and nitrate reduction were also negative. All isolates failed to grow at 37 °C. The DNA G+C content of strain 130333T was 53 mol%. On the basis of data obtained in this study, the six isolates represent a novel species of a new genus in the family Enterobacteriaceae, named Rouxiella chamberiensis gen. nov., sp. nov. The type strain of the type species is 130333T ( = CIP 110714T = DSM 28324T).
研究发现，新生儿肠外营养袋被一种此前未描述过的肠杆菌科成员污染。通过rrs基因（编码16S rRNA）序列分析和多位点序列分析（MLSA）对6株分离菌进行研究，结果显示它们形成了一个独立分支，与尤因氏菌属、拉恩氏菌属、耶尔森氏菌属、哈夫尼菌属和沙雷氏菌属相近。从表型上看，这个新分类单元与这五个菌属不同。该新分类单元在Voges-Proskauer试验、西蒙斯柠檬酸盐试验和邻硝基苯-β-半乳糖苷水解试验中呈阳性；能发酵d-葡萄糖、d-甘露醇、l-鼠李糖、蜜二糖、l-阿拉伯糖和d-木糖；能水解七叶苷；不能发酵麦芽糖、海藻糖、棉子糖、d-山梨醇、蔗糖或纤维二糖。运动性、产气、脲酶、明胶酶和硝酸盐还原试验也均为阴性。所有分离菌在37℃均不能生长。菌株130333T的DNA G+C含量为53 mol%。根据本研究获得的数据，这6株分离菌代表了肠杆菌科一个新属的一个新物种，命名为腔室柔西氏菌（Rouxiella chamberiensis），属名新属，种名新种。模式种的模式菌株为130333T（=CIP 110714T = DSM 28324T）。
