Li Yong, Fang Wei, Xue Han, Liang Wen-Xing, Wang Lai-Fa, Tian Guo-Zhong, Wang Xi-Zhuo, Lin Cai-Li, Li Xia, Piao Chun-Gen
Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, PR China.
Int J Syst Evol Microbiol. 2015 Feb;65(Pt 2):432-437. doi: 10.1099/ijs.0.066068-0. Epub 2014 Nov 10.
Five Gran-stain-negative, facultatively anaerobic, motile, bacterial strains were isolated from symptomatic bark tissue of Populus×euramericana canker. Strains grew at 4-41 °C, pH 4-10 and 0-6 % (w/v) salinity. They were positive with respect to catalase activity and negative for oxidase activity, nitrate reduction and the Voges-Proskauer reaction. Analysis of 16S rRNA gene sequences indicated that these five poplar isolates belong to the genus Brenneria, having highest sequence similarity of 95.98 % with Brenneria goodwinii LMG 26270(T). These five isolates formed a single cluster based on multilocus sequence analysis, indicating that they all belong to a single taxon within the genus Brenneria, which was confirmed by DNA-DNA hybridization. The DNA G+C content was 54.9-55.7 mol%, and the main fatty acids were C16 : 0, C18 : 1ω7c, C17 : 0 cyclo and C16 : 1ω7c/iso-C15 : 0 2-OH. Based on these results, we describe a novel species of the genus Brenneria with the proposed name Brenneria populi sp. nov. The type strain is D9-5(T) ( = CFCC 11963(T) = KCTC 42088(T)).
从欧美杨溃疡病有症状的树皮组织中分离出5株革兰氏阴性、兼性厌氧、具运动性的细菌菌株。这些菌株在4-41℃、pH值4-10和盐度0-6%（w/v）的条件下生长。它们过氧化氢酶活性呈阳性，氧化酶活性、硝酸盐还原和Voges-Proskauer反应呈阴性。16S rRNA基因序列分析表明，这5株杨树分离株属于布伦纳氏菌属，与布伦纳氏菌Brenneria goodwinii LMG 26270(T)的序列相似性最高，为95.98%。基于多位点序列分析，这5株分离株形成了一个单一的聚类，表明它们都属于布伦纳氏菌属内的一个单一分类单元，DNA-DNA杂交证实了这一点。DNA G+C含量为54.9-55.7 mol%，主要脂肪酸为C16 : 0、C18 : 1ω7c、C17 : 0环丙烷和C16 : 1ω7c/异-C15 : 0 2-OH。基于这些结果，我们描述了布伦纳氏菌属的一个新物种，提议命名为杨树布伦纳氏菌（Brenneria populi sp. nov.）。模式菌株为D9-5(T)（=CFCC 11963(T)=KCTC 42088(T)）。
