†Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 905 South Goodwin Avenue, Urbana, Illinois 61801, United States.
§Abbott Nutrition, 3300 Stelzer Road, Columbus, Ohio 43219, United States.
J Agric Food Chem. 2015 Apr 1;63(12):3295-302. doi: 10.1021/jf505721p. Epub 2015 Mar 19.
Human milk oligosaccharides (HMOs) function as prebiotics in the infant gut by selecting for specific species of bifidobacteria and bacteroides, but little is known about their potential utilization by Enterobacteriaceae, the relative numbers of which have been linked to the onset of necrotizing enterocolitis in preterm infants. In this study, the in vitro growth of purified HMOs and other related carbohydrates was evaluated using individual strains of Enterobacteriaceae and an Enterobacteriaceae consortia enriched from piglet feces. None of the Enterobacteriaceae strains grew on 2'-fucosyllactose, 6'-sialyllactose, or lacto-N-neotetraose (LNnT); however, several strains were capable of utilizing galactooligosaccharides, maltodextrin, and the mono- and disaccharide components of HMOs for growth. The enriched fecal consortia also did not grow on 2'-fucosyllactose or 6'-sialyllactose, but there was limited growth on LNnT. It was concluded that 2'-fucosyllactose and 6'-sialyllactose supplementation of preterm infant formula should not lead to an increase in Enterobacteriaceae; however, supplementation with LNnT may require further study.
人乳寡糖 (HMOs) 在婴儿肠道中作为益生元发挥作用，通过选择特定种类的双歧杆菌和拟杆菌，但对于它们被肠杆菌科利用的潜力知之甚少，肠杆菌科的相对数量与早产儿坏死性小肠结肠炎的发病有关。在这项研究中，使用来自仔猪粪便的肠杆菌科单菌株和肠杆菌科混合培养物来评估纯化的 HMO 及其它相关碳水化合物的体外生长情况。没有一种肠杆菌科菌株能够在 2'-岩藻糖基乳糖、6'-唾液酸乳糖或乳-N-新四糖 (LNnT) 上生长；然而，有几个菌株能够利用半乳糖低聚糖、麦芽糊精和 HMO 的单糖和二糖成分进行生长。富集的粪便混合培养物也不能在 2'-岩藻糖基乳糖或 6'-唾液酸乳糖上生长，但在 LNnT 上有有限的生长。因此，结论是在早产儿配方奶粉中添加 2'-岩藻糖基乳糖和 6'-唾液酸乳糖不应导致肠杆菌科的增加；然而，LNnT 的补充可能需要进一步研究。
