Hage David S, Matsuda Ryan
Department of Chemistry, University of Nebraska-Lincoln, 704 Hamilton Hall, 639 N 12 Street, Lincoln, NE, 68588-0304, USA,
Methods Mol Biol. 2015;1286:1-19. doi: 10.1007/978-1-4939-2447-9_1.
Affinity chromatography is one of the most selective and versatile forms of liquid chromatography for the separation or analysis of chemicals in complex mixtures. This method makes use of a biologically related agent as the stationary phase, which provides an affinity column with the ability to bind selectively and reversibly to a given target in a sample. This review examines the early work in this method and various developments that have lead to the current status of this technique. The general principles of affinity chromatography are briefly described as part of this discussion. Past and recent efforts in the generation of new binding agents, supports, and immobilization methods for this method are considered. Various applications of affinity chromatography are also summarized, as well as the influence this field has played in the creation of other affinity-based separation or analysis methods.
亲和色谱法是液相色谱中用于分离或分析复杂混合物中化学物质的最具选择性和通用性的方法之一。该方法利用一种生物相关试剂作为固定相，从而提供一个亲和柱，使其能够选择性地、可逆地结合样品中的特定目标物。本文综述了该方法的早期研究工作以及导致该技术现状的各种发展。作为讨论的一部分，简要描述了亲和色谱法的一般原理。考虑了过去和最近在为该方法生成新的结合剂、载体和固定化方法方面所做的努力。还总结了亲和色谱法的各种应用，以及该领域在创建其他基于亲和的分离或分析方法中所起的作用。
