Quan Run, Wu Xiaolong, Zhang Junjie, Liu Rongxu, Han Jianchun, Liu Danyi
College of Food Science, Northeast Agricultural University, Harbin, China.
Heilongjiang Institute of Green Food Science, Northeast Agricultural University, Harbin, China.
Front Cell Infect Microbiol. 2025 Jun 17;15:1596356. doi: 10.3389/fcimb.2025.1596356. eCollection 2025.
Acrosin is a proteolytic enzyme in the sperm acrosome that can stimulate sperm to penetrate the zona pellucida, causing the fertilization of the oocyte. Its activity is a crucial indicator of the sperm's fertilization ability, which is critical in mammalian and human reproduction. However, there exists a lack of a comprehensive review of acrosin. In this study, we compared the extraction methods of acrosin, including acid extraction, buffer extraction, and saline extraction. The main methods for purifying acrosin, such as ammonium sulfate precipitation, ultrafiltration, dialysis, gel filtration chromatography, ion-exchange chromatography, and affinity chromatography, are reviewed. In addition, a detailed overview of the biological functions, inhibitors and applications of acrosin are outlined. This study provides methods for the extraction and purification of acrosin and some theoretical basis for the study of its properties. This provides a reference for further research on acrosin.
顶体蛋白酶是精子顶体中的一种蛋白水解酶，可刺激精子穿透透明带，使卵母细胞受精。其活性是精子受精能力的关键指标，在哺乳动物和人类生殖中至关重要。然而，目前缺乏对顶体蛋白酶的全面综述。在本研究中，我们比较了顶体蛋白酶的提取方法，包括酸提取、缓冲液提取和盐提取。综述了纯化顶体蛋白酶的主要方法，如硫酸铵沉淀、超滤、透析、凝胶过滤色谱、离子交换色谱和亲和色谱。此外，还概述了顶体蛋白酶的生物学功能、抑制剂和应用的详细情况。本研究提供了顶体蛋白酶的提取和纯化方法以及一些关于其性质研究的理论依据。这为进一步研究顶体蛋白酶提供了参考。
