靶向 RNA 测序的长非编码 RNA 的定量基因谱分析。
Quantitative gene profiling of long noncoding RNAs with targeted RNA sequencing.
机构信息
1] Garvan Institute of Medical Research, Sydney, Australia. [2] MRC Functional Genomics Unit, Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK.
1] Garvan Institute of Medical Research, Sydney, Australia. [2] St Vincents Clinical School, Faculty of Medicine, University of New South Wales, Sydney, Australia.
出版信息
Nat Methods. 2015 Apr;12(4):339-42. doi: 10.1038/nmeth.3321. Epub 2015 Mar 9.
We compared quantitative RT-PCR (qRT-PCR), RNA-seq and capture sequencing (CaptureSeq) in terms of their ability to assemble and quantify long noncoding RNAs and novel coding exons across 20 human tissues. CaptureSeq was superior for the detection and quantification of genes with low expression, showed little technical variation and accurately measured differential expression. This approach expands and refines previous annotations and simultaneously generates an expression atlas.
我们比较了定量 RT-PCR（qRT-PCR）、RNA-seq 和捕获测序（CaptureSeq）在组装和定量 20 个人体组织中的长非编码 RNA 和新型编码外显子方面的能力。对于低表达基因的检测和定量，CaptureSeq 具有优势，表现出较小的技术差异，并能准确测量差异表达。这种方法扩展和完善了以前的注释，同时生成了一个表达图谱。
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