Huang Ling, Han Gang
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Small Methods. 2018 Sep 11;2(9). doi: 10.1002/smtd.201700370. Epub 2018 Jul 25.
Boron dipyrromethene (BODIPY) is a class of important emerging fluorescent dyes. Due to their unique chemical and optical properties, near infrared (NIR)-emitting BODIPY dyes containing nanoparticles have recently been developed for a wide array of cutting-edge cancer optotheranostic applications. These nanoparticles not only have robust photostability and tunable photophysical properties, but they can also be flexibly tailored to a multitude of functional uses. Based on these outstanding characteristics, such nanoparticles have shown great promise in diagnosis as biological sensors, as well as in their utilization in advanced imaging and photomedicine for cancer treatment. In particular, here, this study first discusses their use as photoswitchable fluorescence probes toward in vitro single-molecule imaging. Second, this study takes a look at their opportunities for photoacoustic imaging utilization. Third, approaches are discussed to construct new NIR-absorbing BODIPY nanoparticles for photodynamic therapy (PDT). Fourth, this study delves into the new approach to use such nanoparticles as an emerging version of triplet-triplet annihilation upconversion (TTA-UC) and their biological uses, such as their photoactivation prodrug therapy (PAPT) for cancer. Finally, new biological sensors based on NIR BODIPY nanoparticles are introduced.
硼二吡咯亚甲基（BODIPY）是一类重要的新兴荧光染料。由于其独特的化学和光学性质，近年来已开发出含有纳米颗粒的近红外（NIR）发射BODIPY染料，用于一系列前沿的癌症光诊疗应用。这些纳米颗粒不仅具有强大的光稳定性和可调谐的光物理性质，而且还可以灵活地定制用于多种功能用途。基于这些突出特性，此类纳米颗粒在作为生物传感器的诊断方面，以及在癌症治疗的先进成像和光医学应用中都显示出了巨大的潜力。特别是，本研究首先讨论了它们作为可光开关荧光探针用于体外单分子成像的用途。其次，本研究探讨了它们在光声成像应用方面的机会。第三，讨论了构建用于光动力疗法（PDT）的新型近红外吸收BODIPY纳米颗粒的方法。第四，本研究深入探讨了将此类纳米颗粒用作三重态-三重态湮灭上转换（TTA-UC）的新兴版本及其生物学用途的新方法，例如它们用于癌症的光活化前药疗法（PAPT）。最后，介绍了基于近红外BODIPY纳米颗粒的新型生物传感器。
