Sunarto Agus, McColl Kenneth A
CSIRO Biosecurity Flagship, Australian Animal Health Laboratory, Geelong, VIC 3220, Australia.
Dis Aquat Organ. 2015 Mar 9;113(2):127-35. doi: 10.3354/dao02824.
Fish herpesviruses and their hosts may have coevolved for 400 to 450 million yr. During this coexistence, the hosts have equipped themselves with an elaborate immune system to defend themselves from invading viruses, whereas the viruses have developed strategies to evade host immunity, including the expression of cytokine genes that have been captured from the host. Taking advantage of our experimental model for cyprinid herpesvirus 3 (CyHV-3) persistence in carp, we studied the gene expression of host and virus immune-related genes in each stage of infection: acute, persistent and reactivation phases. IFNγ-1, IFNγ-2, IL-12 and IL-10 host genes, and the CyHV-3 vIL-10 gene (khvIL-10) were highly significantly up-regulated in different phases of CyHV-3 infection. Similarly, host IL-1β was up-regulated in the acute phase of CyHV-3 infection. There was no significant difference in the expression of host TNFα-1 and MHC-II genes during all phases of CyHV-3 infection. Based on the expression profile of carp immune-related genes in each stage of CyHV-3 infection, we propose a possible interaction between carp IL-12, carp IL-10 and khvIL-10 during the course of viral infection. To our knowledge, this is the first report on the expression of cytokine genes during all phases (acute, persistent and reactivation) of CyHV-3 infection.
鱼类疱疹病毒及其宿主可能已经共同进化了4亿到4.5亿年。在这种共存过程中，宿主已经配备了一套复杂的免疫系统来抵御入侵的病毒，而病毒则发展出了逃避宿主免疫的策略，包括表达从宿主捕获的细胞因子基因。利用我们关于鲤疱疹病毒3（CyHV-3）在鲤鱼中持续存在的实验模型，我们研究了感染各阶段（急性、持续和再激活阶段）宿主和病毒免疫相关基因的表达。IFNγ-1、IFNγ-2、IL-12和IL-10宿主基因，以及CyHV-3的vIL-10基因（khvIL-10）在CyHV-3感染的不同阶段均高度显著上调。同样，宿主IL-1β在CyHV-3感染的急性期上调。在CyHV-3感染的所有阶段，宿主TNFα-1和MHC-II基因的表达没有显著差异。基于CyHV-3感染各阶段鲤鱼免疫相关基因的表达谱，我们提出了在病毒感染过程中鲤鱼IL-12、鲤鱼IL-10和khvIL-10之间可能存在的相互作用。据我们所知，这是关于CyHV-3感染所有阶段（急性、持续和再激活）细胞因子基因表达的首次报道。
