Saroj Dina B, Dengeti Shrinivas N, Aher Supriya, Gupta Anil K
Advanced Enzyme Technologies Ltd., 5th Floor, A WING, Sun Magnetica, LIC Service Road, Louiswadi, Thane (W), 400 604, Maharashtra, India,
World J Microbiol Biotechnol. 2015 Jun;31(6):995-9. doi: 10.1007/s11274-015-1839-9. Epub 2015 Mar 10.
Trichoderma species are widely used as production hosts for industrial enzymes. Identification of Trichoderma species requires a complex molecular biology based identification involving amplification and sequencing of multiple genes. Industrial laboratories are required to run identification tests repeatedly in cell banking procedures and also to prove absence of production host in the product. Such demands can be fulfilled by a brief method which enables confirmation of strain identity. This communication describes one step identification method for two common Trichoderma species; T. citrinoviride and T. reesei, based on identification of polymorphic region in the nucleotide sequence of translation elongation factor 1 alpha. A unique forward primer and common reverse primer resulted in 153 and 139 bp amplicon for T. citrinoviride and T. reesei, respectively. Simplification was further introduced by using mycelium as template for PCR amplification. Method described in this communication allows rapid, one step identification of two Trichoderma species.
木霉属物种被广泛用作工业酶的生产宿主。木霉属物种的鉴定需要基于复杂分子生物学的鉴定，包括多个基因的扩增和测序。工业实验室需要在细胞库程序中反复进行鉴定测试，并证明产品中不存在生产宿主。这样的需求可以通过一种能够确认菌株身份的简便方法来满足。本通讯描述了一种针对两种常见木霉属物种（黄绿木霉和里氏木霉）的一步鉴定方法，该方法基于对翻译延伸因子1α核苷酸序列中多态性区域的鉴定。一个独特的正向引物和一个通用的反向引物分别产生了153 bp和139 bp的黄绿木霉和里氏木霉扩增子。通过使用菌丝体作为PCR扩增模板进一步简化了方法。本通讯中描述的方法允许对两种木霉属物种进行快速的一步鉴定。
