Sun An-Qi, Ju Xiu-Lian
Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering & Pharmacy, 34756Wuhan Institute of Technology, Wuhan, People's Republic of China.
The College of Post and Telecommunication, 34756Wuhan Institute of Technology, Wuhan, People's Republic of China.
J Int Med Res. 2020 Nov;48(11):300060520968353. doi: 10.1177/0300060520968353.
We investigated the antitumor effects of salidroside and preliminarily examined its underlying mechanisms by establishing a nude mouse model bearing MCF-7 breast cancer cell xenografts.
The mice were grouped and intraperitoneally injected with salidroside, paclitaxel, or physiological saline. Tumor samples were weighed, and immunohistochemical staining with hematoxylin and eosin and anti-CD34 antibody was performed. Tumor cell apoptosis was observed using the terminal deoxynucleotidyl transferase deoxyuridine dUTP nick end labeling assay. Bcl-1, p53, Bax, and caspase 3 expression in tumor tissues was determined via western blotting.
The tumor inhibition rate of high-dose salidroside was 75.16%, which was significantly higher than the rates for paclitaxel and saline. A tumor tissue pathology analysis revealed that high-dose salidroside inhibited tumor cell proliferation and promoted tumor cell apoptosis. Western blotting revealed that Bcl-2 and p53 expression were significantly lower in the salidroside group than in the other groups, whereas Bax and caspase 3 (17 kDa) expression were increased.
Salidroside was more effective than paclitaxel in inhibiting tumor growth in MCF-7 breast cancer cell-bearing nude mice. The mechanism of action may involve Bcl-2 and p53 downregulation and Bax and caspase 3 upregulation, thereby increasing proapoptotic factor expression and inducing tumor cell apoptosis.
通过建立荷MCF-7乳腺癌细胞异种移植瘤的裸鼠模型，研究红景天苷的抗肿瘤作用并初步探讨其潜在机制。
将小鼠分组，分别腹腔注射红景天苷、紫杉醇或生理盐水。称取肿瘤样本重量，并进行苏木精-伊红染色及抗CD34抗体免疫组化染色。采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法观察肿瘤细胞凋亡情况。通过蛋白质免疫印迹法检测肿瘤组织中Bcl-1、p53、Bax和半胱天冬酶3的表达。
高剂量红景天苷的肿瘤抑制率为75.16%，显著高于紫杉醇组和生理盐水组。肿瘤组织病理学分析显示，高剂量红景天苷可抑制肿瘤细胞增殖并促进肿瘤细胞凋亡。蛋白质免疫印迹法显示，红景天苷组中Bcl-2和p53的表达显著低于其他组，而Bax和半胱天冬酶3（17 kDa）的表达增加。
在荷MCF-7乳腺癌细胞的裸鼠中，红景天苷在抑制肿瘤生长方面比紫杉醇更有效。其作用机制可能涉及下调Bcl-2和p53以及上调Bax和半胱天冬酶3，从而增加促凋亡因子表达并诱导肿瘤细胞凋亡。
