Pitkänen A, Beal M F, Sirviö J, Swartz K J, Männistö P T, Riekkinen P J
Department of Neurology, University of Kuopio, Finland.
Neuropeptides. 1989 Oct;14(3):197-207. doi: 10.1016/0143-4179(89)90045-0.
We studied the effect of pentylenetetrazol (PTZ)-induced kindling (35 mg/kg, i.p., daily) on somatostatin-like immunoreactivity (SOM) with special attention to the duration of changes (rats were sacrificed either 10 days or 4 months after the development of kindling) and to transmitters or modulators related to somatostatin (neuropeptide Y (NPY), GABA, choline acetyltransferase (ChAT), acetylcholinesterase (AchE]. In rats sacrificed 10 days after the last kindled seizure, SOM was elevated in frontal cortex and striatum (p less than 0.01); NPY was elevated in frontal cortex, striatum and hippocampus (p less than 0.05) of kindled or prekindled rats (i.e., rats which were treated daily with PTZ but did not express three consecutive generalized seizures). ChAT activity was slightly decreased (p less than 0.05) in cortex. GABA levels and AchE activity were unchanged in kindled cortex. In rats sacrificed 4 months after the development of kindling none of the parameters analyzed differed from controls. The present study suggests that the cortical and striatal neurons containing SOM/NPY are affected by PTZ-kindling. The cortical cholinergic system is affected to a much smaller extent. The neuropeptide changes are not persistent, as is the lowered seizure threshold, so they are probably not involved in the maintainance of the latter.
我们研究了戊四氮（PTZ）诱导的点燃效应（35毫克/千克，腹腔注射，每日一次）对生长抑素样免疫反应性（SOM）的影响，特别关注变化的持续时间（点燃发作后10天或4个月处死大鼠）以及与生长抑素相关的递质或调质（神经肽Y（NPY）、γ-氨基丁酸（GABA）、胆碱乙酰转移酶（ChAT）、乙酰胆碱酯酶（AchE））。在末次点燃发作后10天处死的大鼠中，点燃或预点燃大鼠（即每日用PTZ处理但未出现连续三次全身性发作的大鼠）的额叶皮质和纹状体中SOM升高（p<0.01）；额叶皮质、纹状体和海马体中的NPY升高（p<0.05）。皮质中ChAT活性略有降低（p<0.05）。点燃皮质中的GABA水平和AchE活性未发生变化。在点燃发作后4个月处死的大鼠中，所分析的参数均与对照组无差异。本研究表明，含有SOM/NPY的皮质和纹状体神经元受PTZ点燃的影响。皮质胆碱能系统受影响的程度要小得多。神经肽的变化并不像降低的癫痫阈值那样持久，因此它们可能不参与后者的维持。
