Allahverdi Amin, Sharifi Davood, Takhtfooladi Mohammad Ashrafzadeh, Hesaraki Saeed, Khansari Mohammadreza, Dorbeh Shahab Sarrout
Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University, Tehran, Iran,
Lasers Med Sci. 2015 May;30(4):1305-13. doi: 10.1007/s10103-015-1733-6. Epub 2015 Mar 11.
Tendon repair is still one of the challenges for rehabilitation. Various treatments for tendon injuries have been used in recent decade. This study was established to investigate the effects of low-level laser therapy (LLLT), platelet-rich plasma (PRP) treatment alone, and using combined method on the healing of Achilles tendon in rabbits. Seventy-two healthy mature male white New Zealand rabbits were divided randomly into four groups of 18 animals each: control: partial tenotomy with no treatment, only 1 mL normal saline was injected on days 1, 8, and 15 at the site of splitting; PRP: partial tenotomy with PRP treatment on days 1, 8, and 15 at the site of splitting; LLLT: partial tenotomy with LLLT (K30 hand-held probe, AZOR, Technica, Russia, 650 nm, 30 mW, surface area = 1 cm(2), 60 S/cm(2), energy density = 1.8 J/cm(2)) for 15 consecutive days; LLLT + PRP: partial tenotomy with LLLT + PRP. At the end of trial, the rabbits were euthanatized and tendon specimens were harvested and were submitted for histopathological evaluation, hydroxyproline levels, and biomechanical measurement. The Tukey post hoc test was performed. The results for these parameters showed that PRP or LLLT alone has significant advantages over untreated animals (P < 0.05). Furthermore, it was found that the combined treatment with PRP and LLLT is even more efficient. There was no significant difference (P > 0.05) between the two groups of LLLT and PRP. However, the treatments combining PRP and LLLT showed significant results in comparison of PRP or LLLT alone (P < 0.05). Our results demonstrate that the healing time of injured tendon decreases by using the two therapies combined.
肌腱修复仍是康复领域面临的挑战之一。近十年来，人们采用了各种治疗肌腱损伤的方法。本研究旨在探讨低强度激光疗法（LLLT）、单纯富血小板血浆（PRP）治疗以及联合使用这两种方法对兔跟腱愈合的影响。将72只健康成熟的雄性新西兰白兔随机分为四组，每组18只：对照组：行部分腱切断术，不进行治疗，仅在第1、8和15天在切开部位注射1毫升生理盐水；PRP组：行部分腱切断术，并在第1、8和15天在切开部位进行PRP治疗；LLLT组：行部分腱切断术，连续15天使用LLLT（K30手持探头，AZOR，Technica，俄罗斯，650纳米，30毫瓦，表面积 = 1平方厘米，60 S/平方厘米，能量密度 = 1.8焦耳/平方厘米）；LLLT + PRP组：行部分腱切断术，并使用LLLT + PRP。试验结束时，对兔子实施安乐死，采集肌腱标本并进行组织病理学评估、羟脯氨酸水平检测和生物力学测量。采用Tukey事后检验。这些参数的结果表明，单独使用PRP或LLLT比未治疗的动物具有显著优势（P < 0.05）。此外，发现PRP和LLLT联合治疗效果更佳。LLLT组和PRP组之间无显著差异（P > 0.05）。然而，与单独使用PRP或LLLT相比，PRP和LLLT联合治疗显示出显著效果（P < 0.05）。我们的结果表明，联合使用这两种疗法可缩短受伤肌腱的愈合时间。
