眼内炎病原体发现的新兴技术。
Emerging techniques for pathogen discovery in endophthalmitis.
作者信息
Hong Bryan K, Lee Cecilia S, Van Gelder Russell N, Garg Sunir J
机构信息
aMid Atlantic Retina, The Retina Service of Wills Eye Hospital, Philadelphia, Pennsylvania bThe Department of Ophthalmology, University of Washington Medical Center, Seattle, Washington, USA.
出版信息
Curr Opin Ophthalmol. 2015 May;26(3):221-5. doi: 10.1097/ICU.0000000000000145.
PURPOSE OF REVIEW
Despite the inability to detect certain organisms and relatively low yield, microbial culture is the current gold standard for the diagnosis of most intraocular infections. Research on alternative molecular diagnostic methods has produced an array of strategies that augment and improve pathogen detection. This review summarizes the most recent literature on this topic.
RECENT FINDINGS
The yield of traditional microbial culture has not improved since the Endophthalmitis Vitrectomy Study results were published 20 years ago. Advances in PCR methods have enabled quantification of pathogen load and screening for multiple organisms at once. More recently, deep sequencing techniques allow highly sensitive detection of any DNA-based life form in a specimen. This offers the promise of not only improved detection of traditional organisms but can also identify organisms not previously associated with endophthalmitis.
SUMMARY
Molecular diagnostic methods enhance the results of microbial culture and may become the new standard in the diagnosis of intraocular infections.
综述目的
尽管微生物培养无法检测某些病原体且检出率相对较低，但它仍是目前诊断大多数眼内感染的金标准。对替代分子诊断方法的研究产生了一系列增强和改进病原体检测的策略。本综述总结了该主题的最新文献。
最新发现
自20年前玻璃体切除术治疗眼内炎研究结果发表以来，传统微生物培养的检出率并未提高。聚合酶链反应（PCR）方法的进展使得能够对病原体载量进行定量，并能同时筛查多种病原体。最近，深度测序技术能够高度灵敏地检测样本中任何基于DNA的生命形式。这不仅有望改进对传统病原体的检测，还能识别以前与眼内炎无关的病原体。
总结
分子诊断方法可提高微生物培养的结果，可能成为眼内感染诊断的新标准。
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