在血培养中，对于酵母菌种的直接基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱鉴定，内部检测法优于Sepsityper法。
An in-house assay is superior to Sepsityper for direct matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry identification of yeast species in blood cultures.
作者信息
Bidart Marie, Bonnet Isabelle, Hennebique Aurélie, Kherraf Zine Eddine, Pelloux Hervé, Berger François, Cornet Muriel, Bailly Sébastien, Maubon Danièle
机构信息
UF Clinatec, Pôle Recherche, CHU de Grenoble, Grenoble, France.
Laboratoire de Parasitologie-Mycologie, Institut de Biologie et de Pathologie, CHU de Grenoble, Grenoble, France.
出版信息
J Clin Microbiol. 2015 May;53(5):1761-4. doi: 10.1128/JCM.03600-14. Epub 2015 Mar 11.
Abstract
We developed an in-house assay for the direct identification, by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry, of yeasts in blood culture. Sixty-one representative strains from 12 species were analyzed in spiked blood cultures. Our assay accurately identified 95 of 107 (88.8%) positive blood cultures and outperformed the commercial Sepsityper kit (81.7% identification).
摘要
我们开发了一种内部检测方法，通过基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱直接鉴定血培养中的酵母。对来自12个物种的61株代表性菌株进行了加标血培养分析。我们的检测方法准确鉴定了107份阳性血培养中的95份（88.8%），性能优于商业Sepsityper试剂盒（鉴定率81.7%）。
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