Westblade Lars F, Garner Omai B, MacDonald Karen, Bradford Constance, Pincus David H, Mochon A Brian, Jennemann Rebecca, Manji Ryhana, Bythrow Maureen, Lewinski Michael A, Burnham Carey-Ann D, Ginocchio Christine C
Department of Pathology, Children's Healthcare of Atlanta, Atlanta, Georgia, USA Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA Department of Pathology and Laboratory Medicine, Washington University in St. Louis School of Medicine, St. Louis, Missouri, USA.
Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA.
J Clin Microbiol. 2015 Jul;53(7):2349-52. doi: 10.1128/JCM.00187-15. Epub 2015 Apr 29.
Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has revolutionized the identification of clinical bacterial and yeast isolates. However, data describing the reproducibility of MALDI-TOF MS for microbial identification are scarce. In this study, we show that MALDI-TOF MS-based microbial identification is highly reproducible and can tolerate numerous variables, including differences in testing environments, instruments, operators, reagent lots, and sample positioning patterns. Finally, we reveal that samples of bacterial and yeast isolates prepared for MALDI-TOF MS identification can be repeatedly analyzed without compromising organism identification.
基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱（MS）彻底改变了临床细菌和酵母菌分离株的鉴定方法。然而，关于MALDI-TOF MS用于微生物鉴定的可重复性的数据却很稀少。在本研究中，我们表明基于MALDI-TOF MS的微生物鉴定具有高度可重复性，并且能够耐受众多变量，包括测试环境、仪器、操作人员、试剂批次以及样品定位模式的差异。最后，我们发现为MALDI-TOF MS鉴定制备的细菌和酵母菌分离株样品可以反复分析而不影响菌种鉴定。
