Ferrario Chiara, Milani Christian, Mancabelli Leonardo, Lugli Gabriele Andrea, Turroni Francesca, Duranti Sabrina, Mangifesta Marta, Viappiani Alice, Sinderen Douwe van, Ventura Marco
Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parco Area delle Scienze 11a, 43124 Parma, Italy.
Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland.
FEMS Microbiol Ecol. 2015 Mar;91(3). doi: 10.1093/femsec/fiv009. Epub 2015 Jan 27.
During recent years, the significant and increasing interest in novel bifidobacterial strains with health-promoting characteristics has catalyzed the development of methods for efficient and reliable identification of Bifidobacterium strains at (sub) species level. We developed an assay based on recently acquired bifidobacterial genomic data and involving 98 primer pairs, called the Bifidobacterium-ampliseq panel. This panel includes multiplex PCR primers that target both core and variable genes of the pangenome of this genus. Our results demonstrate that the employment of the Bifidobacterium-ampliseq panel allows rapid and specific identification of the so far recognized 48 (sub)species harboring the Bifidobacterium genus, and thus represents a cost- and time-effective bifidobacterial screening methodology.
近年来，对具有促进健康特性的新型双歧杆菌菌株的显著且不断增加的关注，推动了在（亚）种水平上高效、可靠地鉴定双歧杆菌菌株方法的发展。我们基于最近获得的双歧杆菌基因组数据开发了一种检测方法，该方法涉及98对引物，称为双歧杆菌扩增子测序面板。该面板包括针对该属泛基因组核心基因和可变基因的多重PCR引物。我们的结果表明，使用双歧杆菌扩增子测序面板能够快速、特异性地鉴定出目前已识别的双歧杆菌属的48个（亚）种，因此代表了一种经济高效的双歧杆菌筛选方法。
