牛角瓜乳胶高分子量蛋白质亚组分在大鼠体内的抗水肿和抗氧化特性
Antiedematogenic and antioxidant properties of high molecular weight protein sub-fraction of Calotropis procera latex in rat.
作者信息
Chaudhary Priyanka, de Araújo Viana Carolina, Ramos Marcio V, Kumar Vijay L
机构信息
Department of Pharmacology, All India Institute of Medical Sciences, New Delhi, India.
Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60451-970 Fortaleza, CE, Brazil.
出版信息
J Basic Clin Pharm. 2015 Mar;6(2):69-73. doi: 10.4103/0976-0105.152098.
OBJECTIVES
The aim was to evaluate the effect of high molecular weight protein fraction of Calotropis procera latex on edema formation and oxidative stress in carrageenan-induced paw inflammation.
METHODS
A sub-plantar injection of carrageenan was given to induce edema in the hind paw of the rat. The inhibitory effect of high molecular weight protein fraction of C. procera latex was evaluated following intravenous administration (5 and 25 mg/kg body weight) and was compared with that of diclofenac given orally (5 mg/kg). The levels of reduced glutathione (GSH), thiobarbituric acid reactive substances (TBARS) and myeloperoxidase (MPO) were measured in the inflamed paw tissue at the end of the study.
RESULTS
The high molecular weight protein fraction obtained from the latex of C. procera produced a dose-dependent inhibition of edema formation that was accompanied by normalization of levels of oxidative stress markers (GSH and TBARS) and MPO, a marker for neutrophils in the paw tissue.
CONCLUSIONS
The high molecular weight protein fraction of C. procera latex ameliorates acute inflammation in the paw through its antioxidant effect.
目的
旨在评估牛角瓜乳胶的高分子量蛋白组分对角叉菜胶诱导的爪部炎症中水肿形成和氧化应激的影响。
方法
通过足底注射角叉菜胶诱导大鼠后爪水肿。静脉注射（5和25毫克/千克体重）后评估牛角瓜乳胶高分子量蛋白组分的抑制作用，并与口服双氯芬酸（5毫克/千克）的作用进行比较。在研究结束时测量炎症爪组织中还原型谷胱甘肽（GSH）、硫代巴比妥酸反应性物质（TBARS）和髓过氧化物酶（MPO）的水平。
结果
从牛角瓜乳胶中获得的高分子量蛋白组分产生了剂量依赖性的水肿形成抑制作用，同时爪组织中氧化应激标志物（GSH和TBARS）水平以及中性粒细胞标志物MPO恢复正常。
结论
牛角瓜乳胶的高分子量蛋白组分通过其抗氧化作用改善爪部急性炎症。
Zotero 插件