Schaafsma H E, Ramaekers F C, van Muijen G N, Ooms E C, Ruiter D J
Department of Pathology, University Hospital Nijmegen, The Netherlands.
Histochemistry. 1989;91(2):151-9. doi: 10.1007/BF00492389.
Cytokeratin expression was studied in the epithelia lining the normal human urine conducting system using immunohistochemistry on frozen sections employing a panel of 14 monoclonal antibodies. Eleven of these anticytokeratin antibodies reacted specifically with one of the 19 human cytokeratin polypeptides. Profound differences were found in the cytokeratin expression patterns between the different types of epithelium in the male and female urinary tract. In the areas showing morphological transitions of transitional epithelium to columnar epithelium and of nonkeratinizing squamous epithelium to keratinizing squamous epithelium gradual shifts of cytokeratin expression patterns were observed, often anticipating the morphological changes. However, also within one type of epithelium, i.e. the transitional epithelium, two different patterns of cytokeratin expression were found. Expression of cytokeratin 7 was homogeneous in the transitional epithelium of renal pelvis and ureter but heterogeneous in the transitional epithelium of the bladder. Furthermore, intraepithelial differences in cytokeratin expression could be shown to be differentiation related. Using a panel of chain-specific monoclonal antibodies to cytokeratins 8 and 18 conformational and/or biochemical changes in the organization of these intermediate filaments were demonstrated upon differentiation in columnar and transitional epithelium.
利用一组14种单克隆抗体，通过对冰冻切片进行免疫组织化学，研究了正常人尿液传导系统内衬上皮中的细胞角蛋白表达。这些抗细胞角蛋白抗体中的11种与19种人类细胞角蛋白多肽之一发生特异性反应。在男性和女性尿路不同类型上皮之间的细胞角蛋白表达模式中发现了显著差异。在显示移行上皮向柱状上皮以及非角化鳞状上皮向角化鳞状上皮形态转变的区域，观察到细胞角蛋白表达模式的逐渐变化，这种变化常常先于形态学改变。然而，即使在一种上皮类型即移行上皮内，也发现了两种不同的细胞角蛋白表达模式。细胞角蛋白7在肾盂和输尿管的移行上皮中表达均匀，但在膀胱的移行上皮中表达不均一。此外，上皮内细胞角蛋白表达的差异可被证明与分化相关。利用一组针对细胞角蛋白8和18的链特异性单克隆抗体，在柱状上皮和移行上皮分化过程中，证明了这些中间丝组织的构象和/或生化变化。
