Portnoy Emma, Nizri Eran, Golenser Jacob, Shmuel Miriam, Magdassi Shlomo, Eyal Sara
Institute for Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University, Jerusalem, Israel; Casali Center for Applied Chemistry, Institute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, Israel.
Department of General Surgery, Tel-Aviv Sourasky Medical Center, Tel-Aviv, Israel.
Nanomedicine. 2015 Jul;11(5):1057-64. doi: 10.1016/j.nano.2015.02.019. Epub 2015 Mar 16.
Intraoperative ureter identification can assist in the prevention of ureteral injury and consequently improve surgery outcomes. Our aim was to take advantage of the altered pharmacokinetics of liposomal indocyanine green (ICG), the only FDA-approved near-infrared (NIR) dye, for imaging of ureters during surgeries. ICG was passively adsorbed to liposomes. NIR whole mice body and isolated tissue imaging were used to study liposomal ICG properties vs. free ICG. In vivo, the urinary bladder could be clearly observed in most of the liposome-treated mice. Liposomal encapsulation of ICG enhanced ureteral emission up to 1.9 fold compared to free ICG (P<0.01). Increase in liposomal micropolarity and microviscosity and differential scanning calorimetry supported ICG localization within the liposomal bilayer. Our findings suggest that liposomal ICG could be utilized for ureteral imaging intra-operatively, thus potentially improving surgical outcomes.
Iatrogenic ureteral injury is a serious complication of abdominal surgery and intra-operative recognition of the ureters is usually the best method of injury prevention. In this article, the authors developed liposomal indocyanine green, which could be excreted via the urinary system and investigated its in-vivo use in mice.
术中识别输尿管有助于预防输尿管损伤，从而改善手术结果。我们的目的是利用脂质体吲哚菁绿（ICG，唯一获美国食品药品监督管理局批准的近红外（NIR）染料）改变的药代动力学，在手术期间对输尿管进行成像。ICG被被动吸附到脂质体上。使用近红外全小鼠身体和离体组织成像来研究脂质体ICG与游离ICG的特性。在体内，在大多数接受脂质体治疗的小鼠中可以清晰观察到膀胱。与游离ICG相比，ICG的脂质体包封使输尿管发射增强了1.9倍（P<0.01）。脂质体微极性和微粘度的增加以及差示扫描量热法支持ICG在脂质体双层内的定位。我们的研究结果表明，脂质体ICG可用于术中输尿管成像，从而有可能改善手术结果。
医源性输尿管损伤是腹部手术的严重并发症，术中识别输尿管通常是预防损伤的最佳方法。在本文中，作者开发了可通过泌尿系统排泄的脂质体吲哚菁绿，并研究了其在小鼠体内的应用。
