Kim Min-Ju, Han Jae-Kwang, Park Jong-Su, Lee Jin-Sung, Lee Soon-Ho, Cho Joon-Il, Kim Keun-Sung
Department of Food Science and Technology, Chung-Ang University, Ansung 456-756, Republic of Korea.
Department of Biological Sciences, Kyonggi University, Suwon 442-760, Republic of Korea.
J Microbiol Biotechnol. 2015 Jun;25(6):872-9. doi: 10.4014/jmb.1502.02003.
Many strains of Bacillus cereus cause gastrointestinal diseases, and the closely related insect pathogen Bacillus thuringiensis has also been involved in outbreaks of diarrhea. The diarrheal diseases are attributed to enterotoxins. Sixteen reference strains of B. cereus and nine commercial and 12 reference strains of B. thuringiensis were screened by PCR for the presence of 10 enterotoxigenic genes (hblA, hblC, hblD, nheA, nheB, nheC, cytK, bceT, entFM, and entS), one emetogenic gene (ces), seven hemolytic genes (hlyA, hlyII, hlyIII, plcA, cerA, cerB, and cerO), and a pleiotropic transcriptional activator gene (plcR). These genes encode various enterotoxins and other virulence factors thought to play a role in infections of mammals. Amplicons were successfully generated from the strains of B. cereus and B. thuringiensis for each of these sequences, except the ces gene. Intriguingly, the majority of these B. cereus enterotoxin genes and other virulence factor genes appeared to be widespread among B. thuringiensis strains as well as B. cereus strains.
许多蜡样芽孢杆菌菌株可引起胃肠道疾病，与之密切相关的昆虫病原体苏云金芽孢杆菌也与腹泻暴发有关。腹泻病归因于肠毒素。通过PCR对16株蜡样芽孢杆菌参考菌株以及9株苏云金芽孢杆菌商业菌株和12株参考菌株进行筛查，检测10个产肠毒素基因（hblA、hblC、hblD、nheA、nheB、nheC、cytK、bceT、entFM和entS）、1个致呕吐基因（ces）、7个溶血基因（hlyA、hlyII、hlyIII、plcA、cerA、cerB和cerO）以及1个多效转录激活基因（plcR）的存在情况。这些基因编码各种肠毒素和其他毒力因子，被认为在哺乳动物感染中起作用。除ces基因外，蜡样芽孢杆菌和苏云金芽孢杆菌菌株均成功扩增出了这些序列中的每一个序列的扩增子。有趣的是，大多数蜡样芽孢杆菌肠毒素基因和其他毒力因子基因似乎在苏云金芽孢杆菌菌株以及蜡样芽孢杆菌菌株中广泛存在。
