Hematology and Oncology Department, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Blood Borne Infections Research Center, Academic Center for Education, Culture and Research (ACECR), Razavi Khorasan, Mashhad, Iran.
Infect Genet Evol. 2018 Apr;59:148-154. doi: 10.1016/j.meegid.2018.02.004. Epub 2018 Feb 7.
Viral hepatitis is considered as a worldwide health problem and hepatitis B virus (HBV) infection is one of the major health concerns which are annually responsible for more than one million deaths. HBV can be classified into at least eight genotypes, A-H and four major subtypes. Predominant HBV genotype in Mediterranean and Middle East countries is genotype D, but there is a few studies have been performed on the HBV genotype in Iran. The genotype characteristic and phylogenetic analyses were determined in chronic HBV patients in the northeast of Iran. First, seventy-eight patients with chronic HBV infection were enrolled. Demographic features were reviewed and sera samples were collected. HBV genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and results were confirmed by sequencing. Finally, a phylogenetic tree was obtained using Geneious software. Sixty-two (79.48%) of patients were males (mean age: 36.82 years). Twelve out of 78 patients (15.4%) were hepatitis B envelope antigen (HBeAg)-reactive. There were no significant differences between the clinical and HBeAg-positive serological data and HBeAb positive individuals. RFLP DNA sequencing and phylogenetic analysis showed that genotype D was the only genotype which observed in Mashhad, northeast of Iran. This is the first report of HBV genotyping in Mashhad. The results revealed that genotype D was the only genotype detected in this area which was consistence with previous studies in the Middle East, Mediterranean countries, southwest and center of Iran.
病毒性肝炎被认为是一个全球性的健康问题，乙型肝炎病毒 (HBV) 感染是主要的健康关注点之一，每年导致超过 100 万人死亡。HBV 至少可分为 8 个基因型，A-H，以及 4 个主要亚型。地中海和中东国家主要的 HBV 基因型为基因型 D，但关于伊朗 HBV 基因型的研究较少。本研究对伊朗东北部慢性 HBV 患者的 HBV 基因型特征和系统进化进行了分析。首先，纳入了 78 例慢性 HBV 感染患者。分析了患者的人口统计学特征，并收集了血清样本。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行 HBV 基因分型，测序结果进行了确认。最后，使用 Geneious 软件构建了系统进化树。62 例（79.48%）患者为男性（平均年龄：36.82 岁）。12 例（15.4%）患者 HBeAg 阳性。临床和 HBeAg 阳性血清学数据与 HBeAb 阳性个体之间无显著差异。RFLP DNA 测序和系统进化分析显示，基因型 D 是伊朗东北部马什哈德唯一观察到的基因型。这是马什哈德 HBV 基因分型的首次报告。研究结果表明，该地区仅检测到基因型 D，与之前在中东、地中海国家、伊朗西南部和中部的研究结果一致。
