Taherkhani Reza, Farshadpour Fatemeh, Makvandi Manoochehr, Hamidifard Mojtaba, Esmailizadeh Mahdi, Ahmadi Bijan, Heidari Hamid
Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran ; Department of Microbiology and Parasitology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, IR Iran.
Department of Microbiology and Parasitology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, IR Iran.
Jundishapur J Microbiol. 2015 Feb 2;8(2):e17458. doi: 10.5812/jjm.17458. eCollection 2015 Feb.
The human cytomegalovirus (HCMV) is a common pathogen which usually remains asymptomatic in the healthy adults; however, it can cause a symptomatic disease in the immunocompromised patients. The risk of infection with HCMV increases in ulcerative colitis (UC) patients as a result of receiving immunosuppressive agents.
This study aimed to determine the prevalence and the glycoprotein B genotypes of HCMV among the patients with HCMV disease superimposed on an UC flare that required hospitalization in Imam Khomeini Hospital in Ahvaz, Iran, during 2010- 2012.
In this case-control study, formalin-fixed paraffin-embedded intestinal tissue samples were taken from 98 patients with UC disease including 53 males and 45 females (mean age ± standard deviation, 38.95 ± 17.93) and 67 control patients with noninflammatory disease who were referred to Imam Khomeini Hospital during 2010-2012. Detection of HCMV genome in intestinal samples was carried out by seminested polymerase chain reaction. Glycoprotein B genotypes were determined by sequencing.
Among 98 patients with UC, only 12 (12.2%) patients were positive for HCMV genome, while the HCMV genome was not detected in any of the controls. (P = 0.002). The distribution of HCMV gB genotypes in 12 CMV-positive UC patients was as follow: gB1, 11 (91.7%) and gB3, 1 (8.3%). The most prevalent genotype in CMV-positive UC patients was gB1.
In this study, high prevalence of 91.7% HCMV gB1 genotype was predominant among HCMV-positive UC patients, which suggests that there might be an association between HCMV gB genotype 1 and UC disease.
人巨细胞病毒（HCMV）是一种常见病原体，在健康成年人中通常无症状；然而，它可在免疫功能低下的患者中引起有症状的疾病。由于接受免疫抑制剂治疗，溃疡性结肠炎（UC）患者感染HCMV的风险增加。
本研究旨在确定2010年至2012年期间在伊朗阿瓦士伊玛目霍梅尼医院因UC病情发作需要住院治疗且合并HCMV疾病的患者中HCMV的流行率和糖蛋白B基因型。
在这项病例对照研究中，从98例UC疾病患者中获取福尔马林固定石蜡包埋的肠道组织样本，其中包括53例男性和45例女性（平均年龄±标准差，38.95±17.93），以及2010年至2012年期间转诊至伊玛目霍梅尼医院的67例非炎性疾病对照患者。通过半巢式聚合酶链反应检测肠道样本中的HCMV基因组。通过测序确定糖蛋白B基因型。
在98例UC患者中，只有12例（12.2%）患者的HCMV基因组呈阳性，而在任何对照患者中均未检测到HCMV基因组。（P = 0.002）。12例CMV阳性UC患者中HCMV gB基因型的分布如下：gB1，11例（91.7%）和gB3，1例（8.3%）。CMV阳性UC患者中最常见的基因型是gB1。
在本研究中，HCMV阳性UC患者中HCMV gB1基因型的高流行率占主导地位，这表明HCMV gB1基因型与UC疾病之间可能存在关联。
