采用高效液相色谱-电喷雾电离-线性离子阱-轨道阱质谱法鉴定红酒提取物样品和斑马鱼胚胎中的酚类化合物。
Identification of phenolic compounds in red wine extract samples and zebrafish embryos by HPLC-ESI-LTQ-Orbitrap-MS.
作者信息
Vallverdú-Queralt Anna, Boix Nuria, Piqué Ester, Gómez-Catalan Jesús, Medina-Remon Alexander, Sasot Gemma, Mercader-Martí Mercè, Llobet Juan M, Lamuela-Raventos Rosa M
机构信息
INRA, UMR1083 Sciences pour l'œnologie, 2 Place Pierre Viala, Montpellier Cedex 34060, France; Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (ciberobn), Instituto de Salud Carlos III, Sinesio Delgado 4, 28029 Madrid, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain.
GRET-CERETOX and Toxicology Unit, Pharmacology and Therapeutical Chemistry, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain.
出版信息
Food Chem. 2015 Aug 15;181:146-51. doi: 10.1016/j.foodchem.2015.02.098. Epub 2015 Feb 25.
The zebrafish embryo is a highly interesting biological model with applications in different scientific fields, such as biomedicine, pharmacology and toxicology. In this study, we used liquid chromatography/electrospray ionisation-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC/ESI-LTQ-Orbitrap-MS) to identify the polyphenol compounds in a red wine extract and zebrafish embryos. Phenolic compounds and anthocyanin metabolites were determined in zebrafish embryos previously exposed to the red wine extract. Compounds were identified by injection in a high-resolution system (LTQ-Orbitrap) using accurate mass measurements in MS, MS(2) and MS(3) modes. To our knowledge, this research constitutes the first comprehensive identification of phenolic compounds in zebrafish by HPLC coupled to high-resolution mass spectrometry.
斑马鱼胚胎是一种极具吸引力的生物学模型，在生物医学、药理学和毒理学等不同科学领域都有应用。在本研究中，我们使用液相色谱/电喷雾电离-线性离子阱四极杆-轨道阱质谱联用仪（HPLC/ESI-LTQ-Orbitrap-MS）来鉴定红酒提取物和斑马鱼胚胎中的多酚化合物。在先前暴露于红酒提取物的斑马鱼胚胎中测定了酚类化合物和花青素代谢物。通过在高分辨率系统（LTQ-Orbitrap）中进样，利用MS、MS(2)和MS(3)模式下的精确质量测量来鉴定化合物。据我们所知，本研究首次通过HPLC与高分辨率质谱联用对斑马鱼中的酚类化合物进行了全面鉴定。
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