Kim Soohyun, Park Young Woo, Lee Euiri, Park Sang Wan, Park Sungwon, Kim Jong Whi, Seong Je Kyung, Seo Kangmoon
Department of Veterinary Clinical Science, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
J Vet Sci. 2015;16(3):349-56. doi: 10.4142/jvs.2015.16.3.349. Epub 2015 Mar 20.
To standardize the corneal haze model in the resection depth and size for efficient corneal haze development, air assisted lamellar keratectomy was performed. The ex vivo porcine corneas were categorized into four groups depending on the trephined depth: 250 µm (G1), 375 µm (G2), 500 µm(G3) and 750 µm (G4). The stroma was equally ablated at the five measurement sites in all groups. Significant differences were observed between the trephined corneal depths for resection and ablated corneal thickness in G1 (p < 0.001). No significant differences were observed between the trephined corneal depth for resection and the ablated corneal thickness in G2, G3, and G4. The resection percentage was similar in all groups after microscopic imaging of corneal sections. Air assisted lamellar keratectomy (AK) and conventional keratectomy (CK) method were applied to six beagles, after which development of corneal haze was evaluated weekly until postoperative day 28. The occurrence of corneal haze in the AK group was significantly higher than that in the CK group beginning 14 days after surgery. Alpha-smooth muscle actin expression was significantly higher in the AK group (p < 0.001) than the CK group. Air assisted lamellar keratectomy was used to achieve the desired corneal thickness after resection and produce sufficient corneal haze.
为了在切除深度和大小方面标准化角膜混浊模型以实现有效的角膜混浊发展，进行了空气辅助板层角膜切除术。根据环钻深度，将离体猪角膜分为四组：250 µm（G1组）、375 µm（G2组）、500 µm（G3组）和750 µm（G4组）。在所有组的五个测量部位对基质进行同等程度的切削。G1组中，切除的环钻角膜深度与切削后的角膜厚度之间观察到显著差异（p < 0.001）。G2组、G3组和G4组中，切除的环钻角膜深度与切削后的角膜厚度之间未观察到显著差异。在对角膜切片进行显微镜成像后，所有组的切除百分比相似。对六只比格犬应用空气辅助板层角膜切除术（AK）和传统角膜切除术（CK）方法，术后每周评估角膜混浊的发展情况，直至术后第28天。术后14天开始，AK组角膜混浊的发生率显著高于CK组。AK组中α-平滑肌肌动蛋白的表达显著高于CK组（p < 0.001）。使用空气辅助板层角膜切除术在切除后达到所需的角膜厚度并产生足够的角膜混浊。
